TY - JOUR
T1 - Rhamnose-binding lectins induce respiratory burst activity in macrophage cells from rainbow trout
AU - Watanabe, Yasuharu
AU - Chang, Yin Hsuan
AU - Nakamura, Osamu
AU - Naganuma, Takako
AU - Ogawa, Tomohisa
AU - Muramoto, Koji
N1 - Funding Information:
Acknowledgments This work was supported by Grants-in-Aid for Scientific Research (23380121) from the Ministry of Education, Science, Sport and Culture of Japan.
PY - 2013/5
Y1 - 2013/5
N2 - Rhamnose-binding lectins (RBLs) have been isolated and characterized from various kinds of fish. RBLs recognize the structures of lipopolysaccharides and lipoteichoic acid on microbes, and have an opsonic effect, suggesting that RBLs play an important role as pattern recognition proteins in innate immunity. However, the function of RBLs in the removal of pathogens from the body has not yet been elucidated. In this study, we investigated the effect of three RBLs-named CSL1, 2, and 3-that were isolated from chum salmon Oncorhynchus keta eggs on the respiratory burst activity in a macrophage cell line. The CSLs induced reactive oxygen species in the peritoneal macrophage cell line from rainbow trout Oncorhynchus mykiss (RTM5). However, this activity was not affected by l-rhamnose or dl-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol, an inhibitor for glucosylceramide synthesis, indicating that the interaction between CSLs and Gb3 on cell surfaces was not involved in the induction of respiratory burst activity.
AB - Rhamnose-binding lectins (RBLs) have been isolated and characterized from various kinds of fish. RBLs recognize the structures of lipopolysaccharides and lipoteichoic acid on microbes, and have an opsonic effect, suggesting that RBLs play an important role as pattern recognition proteins in innate immunity. However, the function of RBLs in the removal of pathogens from the body has not yet been elucidated. In this study, we investigated the effect of three RBLs-named CSL1, 2, and 3-that were isolated from chum salmon Oncorhynchus keta eggs on the respiratory burst activity in a macrophage cell line. The CSLs induced reactive oxygen species in the peritoneal macrophage cell line from rainbow trout Oncorhynchus mykiss (RTM5). However, this activity was not affected by l-rhamnose or dl-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol, an inhibitor for glucosylceramide synthesis, indicating that the interaction between CSLs and Gb3 on cell surfaces was not involved in the induction of respiratory burst activity.
KW - Lectin
KW - Reactive oxygen species
KW - Respiratory burst
KW - Rhamnose-binding lectin
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U2 - 10.1007/s12562-013-0624-7
DO - 10.1007/s12562-013-0624-7
M3 - Article
AN - SCOPUS:84877629366
SN - 0919-9268
VL - 79
SP - 513
EP - 519
JO - Fisheries Science
JF - Fisheries Science
IS - 3
ER -