rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15

Motoo Kitagawa; Shin Takasawa; Norihisa Kikuchi; Takako Itoh; Hiroshi Teraoka; Hiroshi Yamamoto; Hiroshi Okamoto

doi:10.1016/0014-5793(91)80590-Y

rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15

Motoo Kitagawa, Shin Takasawa, Norihisa Kikuchi, Takako Itoh, Hiroshi Teraoka, Hiroshi Yamamoto, Hiroshi Okamoto

HOSP - Surgery

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50 Citations (Scopus)

Abstract

rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.

Original language	English
Pages (from-to)	210-214
Number of pages	5
Journal	FEBS Letters
Volume	283
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)80590-Y
Publication status	Published - 1991 Jun 3

Keywords

Amino acid sequence
N-terminal modification
Rat liver
Ribosomal protein S15
rig
rig protein

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-5793(91)80590-Y

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title = "rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15",

abstract = "rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.",

keywords = "Amino acid sequence, N-terminal modification, Rat liver, Ribosomal protein S15, rig, rig protein",

author = "Motoo Kitagawa and Shin Takasawa and Norihisa Kikuchi and Takako Itoh and Hiroshi Teraoka and Hiroshi Yamamoto and Hiroshi Okamoto",

note = "Funding Information: Acknowledgernenrs: We are indebtedt o Dr. Tatsuo Tanaka, Depart-mento f BiochemistryS, chool of Medicine,U niversityof the Ryukyus for providing the data base and valuablea dvice, and to Drs. Yuji Ikenishi and Nobuo Yoshida for their assistancea nd encouragement. This work has beens upportedi n part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.",

year = "1991",

month = jun,

day = "3",

doi = "10.1016/0014-5793(91)80590-Y",

language = "English",

volume = "283",

pages = "210--214",

journal = "FEBS Letters",

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TY - JOUR

T1 - rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15

AU - Kitagawa, Motoo

AU - Takasawa, Shin

AU - Kikuchi, Norihisa

AU - Itoh, Takako

AU - Teraoka, Hiroshi

AU - Yamamoto, Hiroshi

AU - Okamoto, Hiroshi

N1 - Funding Information: Acknowledgernenrs: We are indebtedt o Dr. Tatsuo Tanaka, Depart-mento f BiochemistryS, chool of Medicine,U niversityof the Ryukyus for providing the data base and valuablea dvice, and to Drs. Yuji Ikenishi and Nobuo Yoshida for their assistancea nd encouragement. This work has beens upportedi n part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.

PY - 1991/6/3

Y1 - 1991/6/3

N2 - rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.

AB - rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.

KW - Amino acid sequence

KW - N-terminal modification

KW - Rat liver

KW - Ribosomal protein S15

KW - rig

KW - rig protein

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U2 - 10.1016/0014-5793(91)80590-Y

DO - 10.1016/0014-5793(91)80590-Y

M3 - Article

C2 - 2044758

AN - SCOPUS:0025757141

SN - 0014-5793

VL - 283

SP - 210

EP - 214

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15

Abstract

Keywords

UN SDGs

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