Role of α-NADH for microsomal ethanol oxidation

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Abstract

α-NADH* * Reduced form of α-isomer of nicotinamide adenine dinucleotide (1, 2). was found to serve as electron donor for microsomal ethanol oxidation of rat liver. Almost no ethanol oxidation was observed with β-NADH. The α-NADH-dependent ethanol oxidation was almost completely inhibited by 0.1 mM cyanide or azide and strongly abolished in the presence of formate. α-NADH-dependent ethanol oxidation was increased by 1 mM SKF-525A, an inhibitor of microsomal mixed-function oxidase, to about 200%. These results suggested that hydrogen peroxide generated from α-NADH and molecular oxygen in microsomes might be a prerequisite step in the over-all reaction, eventually leading to the peroxidatic ethanol oxidation by catalase to acetaldehyde.

Original languageEnglish
Pages (from-to)793-798
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume50
Issue number3
DOIs
Publication statusPublished - 1973 Feb 5

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