TY - JOUR
T1 - Role of endogenous PGE2 in osteoblastic functions of a clonal osteoblast-like cell, MC3T3-E1
AU - Igarashi, K.
AU - Hirafuji, M.
AU - Adachi, H.
AU - Shinoda, H.
AU - Mitani, H.
N1 - Funding Information:
This study was supportedi n part by a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science, and Culture of Japan (No. 01771825).
PY - 1994/4
Y1 - 1994/4
N2 - MC3T3-E1 cells actively synthesized and released prostaglandin E2 (PGE2) during culture. PGE2 release was minimal on day 9 and gradually increased with culture up to day 27. DNA content gradually increased until day 27. Alkaline phosphatase (ALP) activity increased up to day 15 and decreased thereafter. In contrast to the decrease in ALP activity, calcium accumulation increased rapidly after day 21, possibly due to mineralization by the cells. Indomethacin, a cyclooxygenase inhibitor, blocked PGE2 production completely at concentrations higher than 0.3 μmol/L. In the presence of indomethacin (3 μmol/L), DNA content was slightly decreased on day 27. Furthermore, ALP activity on day 15 was greater than that of the control and this high activity was maintained until day 27. However, calcium accumulation was not affected by the addition of indomethacin. These results suggest that endogenous PGE2 down-regulates ALP activity and slightly stimulates the proliferation of MC3T3-E1 cells as an autocrine mediator, although it does not directly influence the cells' mineralizing activity.
AB - MC3T3-E1 cells actively synthesized and released prostaglandin E2 (PGE2) during culture. PGE2 release was minimal on day 9 and gradually increased with culture up to day 27. DNA content gradually increased until day 27. Alkaline phosphatase (ALP) activity increased up to day 15 and decreased thereafter. In contrast to the decrease in ALP activity, calcium accumulation increased rapidly after day 21, possibly due to mineralization by the cells. Indomethacin, a cyclooxygenase inhibitor, blocked PGE2 production completely at concentrations higher than 0.3 μmol/L. In the presence of indomethacin (3 μmol/L), DNA content was slightly decreased on day 27. Furthermore, ALP activity on day 15 was greater than that of the control and this high activity was maintained until day 27. However, calcium accumulation was not affected by the addition of indomethacin. These results suggest that endogenous PGE2 down-regulates ALP activity and slightly stimulates the proliferation of MC3T3-E1 cells as an autocrine mediator, although it does not directly influence the cells' mineralizing activity.
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U2 - 10.1016/0952-3278(94)90140-6
DO - 10.1016/0952-3278(94)90140-6
M3 - Article
C2 - 8022850
AN - SCOPUS:0028176559
SN - 0952-3278
VL - 50
SP - 169
EP - 172
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
IS - 4
ER -