Role of Gbx2 and Otx2 in the formation of cochlear ganglion and endolymphatic duct

Hiromitsu Miyazaki, Toshimitsu Kobayashi, Harukazu Nakamura, Jun Ichi Funahashi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


The boundary of gene expression of transcription factors often plays a role in making a signaling center in development. In the otic vesicle, Gbx2 is expressed in the dorso-medial region including the endolymphatic duct, and Otx2 in the ventral region. Fgf10 is expressed between their expression boundaries, and the cochleovestibular ganglion develops close to the medial side of the Fgf10 expressing domain. Similar expression patterns are observed in the central nervous system, where Otx2 and Gbx2 expression abut at the mid-hindbrain boundary, and the repressive interaction between Otx2 and Gbx2 defines the mid-hindbrain boundary. These analogous expression patterns raise a question about the role of the interaction between Gbx2 and Otx2 in the otic vesicle. To address this, we misexpressed Gbx2 and Otx2 to the otic epithelium. Ectopic Gbx2 expression could repress Otx2 expression and vice versa. In addition, Fgf10 expression was repressed and cochlear ganglion formation was interfered with. Moreover, endolymphatic duct was severely hypomorphic in the Otx2 misexpressing embryos. These results suggest that the interaction between Gbx2 and Otx2 in developing inner ear defines Fgf10 expression domain to induce the cochlear ganglion. It is also suggested that Gbx2 expression is important for the formation of the endolymphatic duct.

Original languageEnglish
Pages (from-to)429-438
Number of pages10
JournalDevelopment Growth and Differentiation
Issue number7
Publication statusPublished - 2006 Sept


  • Chick
  • In ovo electroporation
  • Inner ear
  • Otic vesicle
  • Regionalization

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


Dive into the research topics of 'Role of Gbx2 and Otx2 in the formation of cochlear ganglion and endolymphatic duct'. Together they form a unique fingerprint.

Cite this