Role of peroxidase and chitosan in removing chlorophenols from aqueous solution

Hossein Ganjidoust, Kenji Tatsumi, Shinji Wada, Mitsuo Kawase

Research output: Contribution to journalConference articlepeer-review

37 Citations (Scopus)


Chlorophenols removal from industrial wastewater by horseradish peroxidase and coagulant was investigated. It was found that an enzymatic reaction time of less than one hour was enough for the reaction to reach 95% completion. Chitosan, which is a natural coagulant, was an effective coagulant as compared to mineral coagulants such as aluminum sulfate (ALUM), hexamethylene diamine epichlorohydrin polycondensate (HX), polyacrylamide (PAM), and polyethyleneimine (PEI). A combination of 0.4 U/mL peroxidase to 2 ppm chitosan along with 0.8 mM of hydrogen peroxide resulted in over 95% chlorophenol removal from aqueous solution.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalWater Science and Technology
Issue number10 pt 6
Publication statusPublished - 1996
EventProceedings of the 1996 18th Biennial Conference of the International Association on Water Quality. Part 6 - Singapore, Singapore
Duration: 1996 Jun 231996 Jun 28


  • Chitosan
  • Chlorophenol
  • Coagulant
  • Horseradish peroxidase
  • Hydrogen peroxide
  • Rate of reaction


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