RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathway

Kana Shimada; Makio Saeki; Hiroshi Egusa; Sho Fukuyasu; Yoshiaki Yura; Kazuhiro Iwai; Yoshinori Kamisaki

doi:10.1016/j.bbrc.2010.12.071

RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathway

Kana Shimada, Makio Saeki, Hiroshi Egusa, Sho Fukuyasu, Yoshiaki Yura, Kazuhiro Iwai, Yoshinori Kamisaki

DEN - Dental Sciences

Osaka University

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Activation of anti-apoptotic gene transcription by NF-κB (nuclear factor-kappa B) has been reported to be linked with a resistance of cancer cells against chemotherapy. NEMO (NF-κB essential modulator) interacts with a number of proteins and modulates the activity of NF-κB pathway. In this study, we revealed that RPAP3 (RNA polymerase II-associated protein 3) possesses an activity to bind with NEMO and to inhibit the ubiquitination of NEMO and that RPAP3 enhances doxorubicin-induced cell death in breast cancer cell line T-47D through the marked impairment of NF-κB pathway. These results indicate that RPAP3 may be a novel modulator of NF-κB pathway in apoptosis induced by anti-cancer chemotherapeutic agents.

Original language	English
Pages (from-to)	910-914
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	404
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2010.12.071
Publication status	Published - 2011 Jan 28

Keywords

Monad
PIH1D1
R2TP
Reptin
RPAP3
WDR92

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2010.12.071

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title = "RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathway",

abstract = "Activation of anti-apoptotic gene transcription by NF-κB (nuclear factor-kappa B) has been reported to be linked with a resistance of cancer cells against chemotherapy. NEMO (NF-κB essential modulator) interacts with a number of proteins and modulates the activity of NF-κB pathway. In this study, we revealed that RPAP3 (RNA polymerase II-associated protein 3) possesses an activity to bind with NEMO and to inhibit the ubiquitination of NEMO and that RPAP3 enhances doxorubicin-induced cell death in breast cancer cell line T-47D through the marked impairment of NF-κB pathway. These results indicate that RPAP3 may be a novel modulator of NF-κB pathway in apoptosis induced by anti-cancer chemotherapeutic agents.",

keywords = "Monad, PIH1D1, R2TP, Reptin, RPAP3, WDR92",

author = "Kana Shimada and Makio Saeki and Hiroshi Egusa and Sho Fukuyasu and Yoshiaki Yura and Kazuhiro Iwai and Yoshinori Kamisaki",

note = "Funding Information: This study was supported in part by Japan Society for the Promotion of Science Grants C20592172 (M.S.), B20390471 (Y.K.), and B22390364 (H.E.) and the Osaka Medical Research Foundation for Incurable Diseases .",

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doi = "10.1016/j.bbrc.2010.12.071",

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T1 - RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathway

AU - Shimada, Kana

AU - Saeki, Makio

AU - Egusa, Hiroshi

AU - Fukuyasu, Sho

AU - Yura, Yoshiaki

AU - Iwai, Kazuhiro

AU - Kamisaki, Yoshinori

N1 - Funding Information: This study was supported in part by Japan Society for the Promotion of Science Grants C20592172 (M.S.), B20390471 (Y.K.), and B22390364 (H.E.) and the Osaka Medical Research Foundation for Incurable Diseases .

PY - 2011/1/28

Y1 - 2011/1/28

N2 - Activation of anti-apoptotic gene transcription by NF-κB (nuclear factor-kappa B) has been reported to be linked with a resistance of cancer cells against chemotherapy. NEMO (NF-κB essential modulator) interacts with a number of proteins and modulates the activity of NF-κB pathway. In this study, we revealed that RPAP3 (RNA polymerase II-associated protein 3) possesses an activity to bind with NEMO and to inhibit the ubiquitination of NEMO and that RPAP3 enhances doxorubicin-induced cell death in breast cancer cell line T-47D through the marked impairment of NF-κB pathway. These results indicate that RPAP3 may be a novel modulator of NF-κB pathway in apoptosis induced by anti-cancer chemotherapeutic agents.

AB - Activation of anti-apoptotic gene transcription by NF-κB (nuclear factor-kappa B) has been reported to be linked with a resistance of cancer cells against chemotherapy. NEMO (NF-κB essential modulator) interacts with a number of proteins and modulates the activity of NF-κB pathway. In this study, we revealed that RPAP3 (RNA polymerase II-associated protein 3) possesses an activity to bind with NEMO and to inhibit the ubiquitination of NEMO and that RPAP3 enhances doxorubicin-induced cell death in breast cancer cell line T-47D through the marked impairment of NF-κB pathway. These results indicate that RPAP3 may be a novel modulator of NF-κB pathway in apoptosis induced by anti-cancer chemotherapeutic agents.

KW - Monad

KW - PIH1D1

KW - R2TP

KW - Reptin

KW - RPAP3

KW - WDR92

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U2 - 10.1016/j.bbrc.2010.12.071

DO - 10.1016/j.bbrc.2010.12.071

M3 - Article

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AN - SCOPUS:79151478266

SN - 0006-291X

VL - 404

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathway

Abstract

Keywords

UN SDGs

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