TY - JOUR
T1 - S1P-dependent interorgan trafficking of group 2 innate lymphoid cells supports host defense
AU - Huang, Yuefeng
AU - Mao, Kairui
AU - Chen, Xi
AU - Sun, Ming An
AU - Kawabe, Takeshi
AU - Li, Weizhe
AU - Usher, Nicholas
AU - Zhu, Jinfang
AU - Urban, Joseph F.
AU - Paul, William E.
AU - Germain, Ronald N.
N1 - Funding Information:
We thank K. Weng, T. Moyer, and C. Henry for cell sorting; A. Moseman for technical assistance with parabiosis surgery; M. Wong for assistance with library preparation; J. M. Ward for analyzing histological sections; and the staff of the NIAID animal facility for the postoperative care of parabiotic mice. We thank E. Shevach and members of the Laboratories of Immunology and Systems Biology, NIAID, for discussions. This work was supported by the Intramural Research Program of NIAID, NIH, and by the USDA (8040-51000-058-00D). Y. Huang was also supported by a NIAID K99 award (1K99AI123350-01A1). RNA-seq data are available in the Gene Expression Omnibus database (accession number GSE104708). Y.F. designed, performed, and interpreted the majority of the experiments and drafted the manuscript. K.M. designed, performed, and interpreted confocal imaging experiments. X.C., T.K., and N.U. assisted with experiments. M.S. performed RNA-seq data analysis. W.L. assisted with image data analysis. J.Z. helped to design and interpret the experiments. J.F.U. provided N. brasiliensis and helped to design and interpret worm experiments. W.E.P. designed the experiments. R.N.G. designed the experiments, interpreted the data, and finalized the manuscript. All authors (with exception of W.E.P.) contributed to the discussion of experimental findings and preparation of the manuscript.
PY - 2018/1/5
Y1 - 2018/1/5
N2 - Innate lymphoid cells (ILCs) are innate counterparts of adaptive T lymphocytes, contributing to host defense, tissue repair, metabolic homeostasis, and inflammatory diseases. ILCs have been considered to be tissue-resident cells, but whether ILCs move between tissue sites during infection has been unclear.We show here that interleukin-25-or helminth-induced inflammatory ILC2s are circulating cells that arise from resting ILC2s residing in intestinal lamina propria. They migrate to diverse tissues based on sphingosine 1-phosphate (S1P)-mediated chemotaxis that promotes lymphatic entry, blood circulation, and accumulation in peripheral sites, including the lung, where they contribute to anti-helminth defense and tissue repair.This ILC2 expansion and migration is a behavioral parallel to the antigen-driven proliferation and migration of adaptive lymphocytes to effector sites and indicates that ILCs complement adaptive immunity by providing both local and distant tissue protection during infection.
AB - Innate lymphoid cells (ILCs) are innate counterparts of adaptive T lymphocytes, contributing to host defense, tissue repair, metabolic homeostasis, and inflammatory diseases. ILCs have been considered to be tissue-resident cells, but whether ILCs move between tissue sites during infection has been unclear.We show here that interleukin-25-or helminth-induced inflammatory ILC2s are circulating cells that arise from resting ILC2s residing in intestinal lamina propria. They migrate to diverse tissues based on sphingosine 1-phosphate (S1P)-mediated chemotaxis that promotes lymphatic entry, blood circulation, and accumulation in peripheral sites, including the lung, where they contribute to anti-helminth defense and tissue repair.This ILC2 expansion and migration is a behavioral parallel to the antigen-driven proliferation and migration of adaptive lymphocytes to effector sites and indicates that ILCs complement adaptive immunity by providing both local and distant tissue protection during infection.
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U2 - 10.1126/science.aam5809
DO - 10.1126/science.aam5809
M3 - Article
C2 - 29302015
AN - SCOPUS:85040125432
SN - 0036-8075
VL - 359
SP - 114
EP - 119
JO - Science
JF - Science
IS - 6371
ER -