Scanning electrochemical microscopy-based drug sensitivity test for a cell culture integrated in silicon microstructures

Yu suke Torisawa; Takatoshi Kaya; Yuki Takii; Daisuke Oyamatsu; Matsuhiko Nishizawa; Tomokazu Matsue

doi:10.1021/ac026317u

Scanning electrochemical microscopy-based drug sensitivity test for a cell culture integrated in silicon microstructures

Yu suke Torisawa, Takatoshi Kaya, Yuki Takii, Daisuke Oyamatsu, Matsuhiko Nishizawa, Tomokazu Matsue

Tohoku University

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

The respiratory activity of collagen-embedded living cells was imaged by scanning electrochemical microscopy (SECM) with the objective to study anticancer drug sensitivity. Two kinds of cancer cells, the human erythroleukemia cell line (K562) and its adriamycin-resistant subline (K562/ADM), were immobilized at the array of microholes micromachined on a silicon wafer for comparative characterization of their sensitivity to the anticancer drug, ADM. The results obtained by the SECM method showed correspondence to a conventional colorimetric assay (SDI assay). Furthermore, since the SECM assay is based on the noninvasive measurement of the respiration activity, continuous monitoring of a dose response was possible.

Original language	English
Pages (from-to)	2154-2158
Number of pages	5
Journal	Analytical Chemistry
Volume	75
Issue number	9
DOIs	https://doi.org/10.1021/ac026317u
Publication status	Published - 2003 May 1

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abstract = "The respiratory activity of collagen-embedded living cells was imaged by scanning electrochemical microscopy (SECM) with the objective to study anticancer drug sensitivity. Two kinds of cancer cells, the human erythroleukemia cell line (K562) and its adriamycin-resistant subline (K562/ADM), were immobilized at the array of microholes micromachined on a silicon wafer for comparative characterization of their sensitivity to the anticancer drug, ADM. The results obtained by the SECM method showed correspondence to a conventional colorimetric assay (SDI assay). Furthermore, since the SECM assay is based on the noninvasive measurement of the respiration activity, continuous monitoring of a dose response was possible.",

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AU - Torisawa, Yu suke

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AU - Oyamatsu, Daisuke

AU - Nishizawa, Matsuhiko

AU - Matsue, Tomokazu

PY - 2003/5/1

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N2 - The respiratory activity of collagen-embedded living cells was imaged by scanning electrochemical microscopy (SECM) with the objective to study anticancer drug sensitivity. Two kinds of cancer cells, the human erythroleukemia cell line (K562) and its adriamycin-resistant subline (K562/ADM), were immobilized at the array of microholes micromachined on a silicon wafer for comparative characterization of their sensitivity to the anticancer drug, ADM. The results obtained by the SECM method showed correspondence to a conventional colorimetric assay (SDI assay). Furthermore, since the SECM assay is based on the noninvasive measurement of the respiration activity, continuous monitoring of a dose response was possible.

AB - The respiratory activity of collagen-embedded living cells was imaged by scanning electrochemical microscopy (SECM) with the objective to study anticancer drug sensitivity. Two kinds of cancer cells, the human erythroleukemia cell line (K562) and its adriamycin-resistant subline (K562/ADM), were immobilized at the array of microholes micromachined on a silicon wafer for comparative characterization of their sensitivity to the anticancer drug, ADM. The results obtained by the SECM method showed correspondence to a conventional colorimetric assay (SDI assay). Furthermore, since the SECM assay is based on the noninvasive measurement of the respiration activity, continuous monitoring of a dose response was possible.

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Scanning electrochemical microscopy-based drug sensitivity test for a cell culture integrated in silicon microstructures

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