Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis

Tetsuyuki Kitamoto; Kohtaro Hikita; Takatoshi Tashima; Jun Tateishi; Yuji Sato

doi:10.1007/BF01116137

Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis

Tetsuyuki Kitamoto, Kohtaro Hikita, Takatoshi Tashima, Jun Tateishi, Yuji Sato

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.

Original language	English
Pages (from-to)	459-465
Number of pages	7
Journal	Bioscience Reports
Volume	6
Issue number	5
DOIs	https://doi.org/10.1007/BF01116137
Publication status	Published - 1986 May 1
Externally published	Yes

Keywords

Alzheimer's disease
SAF
amyloid fibril protein
systemic amyloidosis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1007/BF01116137

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title = "Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis",

abstract = "We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.",

keywords = "Alzheimer's disease, SAF, amyloid fibril protein, systemic amyloidosis",

author = "Tetsuyuki Kitamoto and Kohtaro Hikita and Takatoshi Tashima and Jun Tateishi and Yuji Sato",

year = "1986",

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doi = "10.1007/BF01116137",

language = "English",

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T1 - Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis

AU - Kitamoto, Tetsuyuki

AU - Hikita, Kohtaro

AU - Tashima, Takatoshi

AU - Tateishi, Jun

AU - Sato, Yuji

PY - 1986/5/1

Y1 - 1986/5/1

N2 - We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.

AB - We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.

KW - Alzheimer's disease

KW - SAF

KW - amyloid fibril protein

KW - systemic amyloidosis

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JO - Bioscience Reports

JF - Bioscience Reports

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ER -

Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis

Abstract

Keywords

ASJC Scopus subject areas

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