@inbook{a38ce18dded941ea9919ca696266462f,
title = "Screening and characterization of immunobiotic lactic acid bacteria with porcine immunoassay systems",
abstract = "Microorganisms with the ability to modulate the immune system (immunobiotics) have shown to interact with different pattern recognition receptors (PRRs) expressed in nonimmune and immune cells and exert beneficial effects on host{\textquoteright}s health maintenance and promotion. Suitable assay systems are necessary for an efficient and rapid screening of potential immunobiotic strains. More than a decade of research have allowed us to develop efficient in vitro models based on porcine receptors and cells (porcine immunoassay systems) to study the immunomodulatory effects of lactic acid bacteria (LAB). In addition, detailed studies of model immunobiotic LAB strains with proved abilities to improve immune health in humans (Lactobacillus rhamnosus CRL1505) or pigs (Lactobacillus jensenii TL2937) allowed us to select the most suitable biomarkers that have to be evaluated in those porcine immunoassay systems. Our in vitro models based on transfectant cells expressing porcine PRRs as well as an originally established porcine intestinal epitheliocyte (PIE) cell line have shown to be useful in vitro tools for the selection of immunobiotics and for obtaining information to elucidate the molecular mechanisms behind their beneficial effects.",
keywords = "Immunoassay systems, Immunobiotics, Lactic acid bacteria, Pattern recognition receptors, PIE cells",
author = "Valeria Garcia-Castillo and Leonardo Albarracin and Haruki Kitazawa and Julio Villena",
note = "Funding Information: This study was supported by a Grant-in-Aid for Scientific Research (B) (2) (24380146, 16H05019), Challenging Exploratory Research (23658216, 26660216, 16 K15028), and Open Partnership Joint Projects of JSPS Bilateral Joint Research Projects from the Japan Society for the Promotion of Science (JSPS) to HK and by an ANPCyT-FONCyT Grant PICT-2013 (No. 3219) to JV. This work was also supported by JSPS Core-to-Core Program A (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety.”. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-8907-2_12",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "131--144",
booktitle = "Methods in Molecular Biology",
}