Screening of DNA aptamers against multiple proteins in tissue.

Takahisa Noma; Kazunori Ikebukuro; Koji Sode; Takuya Ohkubo; Yuji Sakasegawa; Naomi Hachiya; Kiyotoshi Kaneko

doi:10.1093/nass/49.1.357

Screening of DNA aptamers against multiple proteins in tissue.

Takahisa Noma, Kazunori Ikebukuro, Koji Sode, Takuya Ohkubo, Yuji Sakasegawa, Naomi Hachiya, Kiyotoshi Kaneko

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We report the development of a novel screening method for DNA aptamers against multiple proteins in the target tissue. Although a purified single protein is generally used in screening of aptamers at present, such a simultaneous selection would be very advantageous in efficiency and selectivity. We first carried out the screening in situ and it was suggested that aptamers against particular target proteins were enriched. We also developed another novel screening method named Aptamer Blotting based on protein separation by PAGE and its visualization with fluorescein labeled aptamers and it was demonstrated that this method allowed the simultaneous selection of specific aptamers against multiple proteins.

Original language	English
Pages (from-to)	357-358
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	49
DOIs	https://doi.org/10.1093/nass/49.1.357
Publication status	Published - 2005
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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AU - Noma, Takahisa

AU - Ikebukuro, Kazunori

AU - Sode, Koji

AU - Ohkubo, Takuya

AU - Sakasegawa, Yuji

AU - Hachiya, Naomi

AU - Kaneko, Kiyotoshi

PY - 2005

Y1 - 2005

N2 - We report the development of a novel screening method for DNA aptamers against multiple proteins in the target tissue. Although a purified single protein is generally used in screening of aptamers at present, such a simultaneous selection would be very advantageous in efficiency and selectivity. We first carried out the screening in situ and it was suggested that aptamers against particular target proteins were enriched. We also developed another novel screening method named Aptamer Blotting based on protein separation by PAGE and its visualization with fluorescein labeled aptamers and it was demonstrated that this method allowed the simultaneous selection of specific aptamers against multiple proteins.

AB - We report the development of a novel screening method for DNA aptamers against multiple proteins in the target tissue. Although a purified single protein is generally used in screening of aptamers at present, such a simultaneous selection would be very advantageous in efficiency and selectivity. We first carried out the screening in situ and it was suggested that aptamers against particular target proteins were enriched. We also developed another novel screening method named Aptamer Blotting based on protein separation by PAGE and its visualization with fluorescein labeled aptamers and it was demonstrated that this method allowed the simultaneous selection of specific aptamers against multiple proteins.

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JO - Nucleic acids symposium series (2004)

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Screening of DNA aptamers against multiple proteins in tissue.

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