Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae

Kozo Tsuchiya, Katsuya Gomi, Katsuhiko Kitamoto, Chieko Kumagai, Gakuzo Tamura

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50 Citations (Scopus)


Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07- 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalApplied Microbiology and Biotechnology
Issue number2-3
Publication statusPublished - 1993 Nov


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