TY - JOUR
T1 - Segregation of Germ Layer Fates by Nuclear Migration-Dependent Localization of Not mRNA
AU - Takatori, Naohito
AU - Kumano, Gaku
AU - Saiga, Hidetoshi
AU - Nishida, Hiroki
N1 - Funding Information:
The authors thank their colleagues in Hiroki Nishida's lab, Hidehiko Hashimoto and Dr. Takefumi Negishi for technical assistance. We thank Dr. Atsuo Nishino and Dr. Hitoyoshi Yasuo for comments and discussions. Animal support was provided by the staffs of Otsuchi Marine Research Center, Tokyo University, Seto Marine Biological Laboratory Field Science Education and Research Center, Kyoto University, and Asamushi Marine Biological Station, Tohoku University. This work was supported by the MEXT, Japan (G.K., H.S., and H.N.) and by JSPS, Japan (N.T. and H.N.).
PY - 2010/10/19
Y1 - 2010/10/19
N2 - An important step in early embryonic development is the allocation and segregation of germ layer fates into distinct embryonic regions. However, the mechanism that segregates the mesendoderm into mesoderm and endoderm fates remains largely unknown in most animals. Here, using ascidians, a primitive chordate, we show that these fates are segregated by partitioning of asymmetrically localized Not mRNA from the mesendoderm cell to its mesodermal daughter. Migration of the mesendoderm cell nucleus to the future mesoderm-forming region, release of Not mRNA from the nucleus, Wnt5α-dependent local retention of the mRNA, and subsequent repositioning of the mitotic spindle to the center of the cell are each required for the asymmetric localization and partitioning of Not mRNA. Our results show that nuclear migration plays an unexpected role in asymmetric cell divisions that segregate germ layer fates in chordate embryos.
AB - An important step in early embryonic development is the allocation and segregation of germ layer fates into distinct embryonic regions. However, the mechanism that segregates the mesendoderm into mesoderm and endoderm fates remains largely unknown in most animals. Here, using ascidians, a primitive chordate, we show that these fates are segregated by partitioning of asymmetrically localized Not mRNA from the mesendoderm cell to its mesodermal daughter. Migration of the mesendoderm cell nucleus to the future mesoderm-forming region, release of Not mRNA from the nucleus, Wnt5α-dependent local retention of the mRNA, and subsequent repositioning of the mitotic spindle to the center of the cell are each required for the asymmetric localization and partitioning of Not mRNA. Our results show that nuclear migration plays an unexpected role in asymmetric cell divisions that segregate germ layer fates in chordate embryos.
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U2 - 10.1016/j.devcel.2010.09.003
DO - 10.1016/j.devcel.2010.09.003
M3 - Article
C2 - 20951349
AN - SCOPUS:77957881083
SN - 1534-5807
VL - 19
SP - 589
EP - 598
JO - Developmental Cell
JF - Developmental Cell
IS - 4
ER -