@article{2d5476fe1d0941a0859ada9ca086ab1a,
title = "Selective formation of a complex of polyadenylate with an impurity in higher plant nucleic acid preparations",
abstract = "Phenol-preparations of higher plant nucleic acids generally resulted in severe contamination by an impurity bound selectively to synthetic polyadenylate (poly(A)). The poly(A) was rapidly bound into a form that was resistant to RNase T2. The impurity sedimented at about 14 S in a sucrose density gradient and had a bouyant density of 1.61 g/cm3 resembling that of polysaccharide. Natural poly(A)-containing RNA also was bound effectively to this impurity.",
keywords = "Contaminant, Hydrophobic binding of poly(A), Poly(A)-containing mRNA, Proteoglycan, Purification of mRNA",
author = "Takeshi Nitta and Hideki Takahashi and Koh Kobayashi and Keisuke Fukuei",
note = "Funding Information: Poly(A) is attached to the 3'-OH end of many mRNAs in eukaryotic cells. The poly(A)-segment is used for identifying and purifying mRNA. On the other hand, several kinds of uridylate-rich RNA have been found to play important roles in the cells \[1--3\].T he content of oligo(U)-containing RNA in *This research was supported in part by a Grant-in-Aid to T.N. from the Ministry of Education, Science and Culture, Japan. **Present address: Laboratory of Plant Pathology, Faculty of Agriculture, Tohoku University, Sendal 980, Japan. Abbreviations: poly(A), polyadenylate; poly(U), polyuridylate; oligo(dT), oligodeoxythy-midylate; SSC, 0.15 M sodium chloride and 0.015 M sodium citrate. Dilutions are given as multiples of this.",
year = "1984",
month = apr,
doi = "10.1016/0304-4211(84)90136-6",
language = "English",
volume = "34",
pages = "135--144",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "1-2",
}