TY - JOUR
T1 - Selective inactivation of elongation factor-2 (EF-2) in free adipocytes obtained by treating Sarcophaga larval fat bodies with chymotrypsin
AU - Okuyama, Hajime
AU - Kurata, Shoichiro
AU - Homma, Ko Ichi
AU - Natori, Shunji
N1 - Funding Information:
The authors are grateful to Dr. C. Brack and Dr. A.C. Nairn for providing antibodies against EF-1α and EF-2, respectively. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Sports, Science and Culture of Japan and CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation (JST).
PY - 1998/5
Y1 - 1998/5
N2 - The molecular mechanism(s) involved in the selective cessation of protein synthesis in free adipocytes obtained by treating fat bodies of Sarcophaga larvae with chymotrypsin were investigated (Okuyama, H., Kurata, S., Natori, S., 1994. Insect Biochem. Mol. Biol. 24, 525-530). Ribosomes prepared from these adipocytes were normal, but the activity of soluble factor(s) needed for protein synthesis was greatly reduced compared with the control fat bodies. From experiments using diphtheria toxin, we concluded that EF-2, but not other factors, was selectively inactivated in these free adipocytes.
AB - The molecular mechanism(s) involved in the selective cessation of protein synthesis in free adipocytes obtained by treating fat bodies of Sarcophaga larvae with chymotrypsin were investigated (Okuyama, H., Kurata, S., Natori, S., 1994. Insect Biochem. Mol. Biol. 24, 525-530). Ribosomes prepared from these adipocytes were normal, but the activity of soluble factor(s) needed for protein synthesis was greatly reduced compared with the control fat bodies. From experiments using diphtheria toxin, we concluded that EF-2, but not other factors, was selectively inactivated in these free adipocytes.
KW - Adipocyte
KW - EF-2
KW - Fat body
KW - Protein synthesis
KW - Sarcophaga peregrina
KW - Tissue remodeling
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U2 - 10.1016/S0965-1748(98)00001-0
DO - 10.1016/S0965-1748(98)00001-0
M3 - Article
AN - SCOPUS:0032078502
SN - 0965-1748
VL - 28
SP - 301
EP - 307
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 5-6
ER -