TY - JOUR
T1 - Sequence determination of peptide by the combined use of fluoresceinisothiocyanate and phenylisothiocyanate
AU - Muramoto, Koji
AU - Kawauchi, Hiroshi
AU - Tuzimura, Katura
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1978/8
Y1 - 1978/8
N2 - A fluorometric manual technique has been demonstrated for determining the amino acid sequence of a polypeptide, by the combined use of fluoresceinisothiocyanate (FITC) and phenylisothiocyanate (pITC). This method permits continuous assignments of 10 residues with 5.6 nmoles of myoglobin or 23 residues with 45 nmoles.
AB - A fluorometric manual technique has been demonstrated for determining the amino acid sequence of a polypeptide, by the combined use of fluoresceinisothiocyanate (FITC) and phenylisothiocyanate (pITC). This method permits continuous assignments of 10 residues with 5.6 nmoles of myoglobin or 23 residues with 45 nmoles.
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U2 - 10.1080/00021369.1978.10863204
DO - 10.1080/00021369.1978.10863204
M3 - Article
AN - SCOPUS:0042490199
SN - 0916-8451
VL - 42
SP - 1559
EP - 1563
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 8
ER -