TY - JOUR
T1 - Seroprevalence of chronic hepatitis B, as determined from dried blood spots, among children and their mothers in central Lao People's Democratic Republic
T2 - A multistage, stratified cluster sampling survey
AU - Komada, Kenichi
AU - Sugiyama, Masaya
AU - Vongphrachanh, Phengta
AU - Xeuatvongsa, Anonh
AU - Khamphaphongphane, Bouaphan
AU - Kitamura, Tomomi
AU - Kiyohara, Tomoko
AU - Wakita, Takaji
AU - Oshitani, Hitoshi
AU - Hachiya, Masahiko
N1 - Funding Information:
Funding source: This work was supported by a Grant for The National Centre for Global Health and Medicine (22-7 and 27-4). The funding source was not involved in the study design, in the collection, analysis and interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.
Publisher Copyright:
© 2015 The Authors.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Background: There is limited information regarding the prevalence of hepatitis B in Lao PDR, where the hepatitis disease burden is substantial. Thus, reliable seroprevalence data is needed for the disease, based on probability sampling. Methods: A stratified, multistage, cluster sampling survey of hepatitis B surface antigen (HBsAg) positivity among children aged 5-9 years and their mothers aged 15-45 years was conducted. Participants were selected randomly from the central region of Lao PDR via probability-proportional-to-size sampling. Blood samples were collected onto filter paper and subsequently analyzed using a chemiluminescent microparticle immunoassay. Results: A total of 911 mother-and-child pairs were collected; the seroprevalence of HBsAg was estimated to be 2.1% (95% confidence interval 0.8-3.4%) among children and 4.1% (95% confidence interval 2.6-5.5%) in their mothers after taking into account the sampling design and the weight of each sample. The children's HBsAg positivity was positively associated with maternal infection and being born in a non-health facility, while the maternal infection status was not associated with any background characteristic. Conclusions: Lao PDR has a relatively lower HBsAg prevalence in the general population compared to surrounding countries. To ensure comparability to other countries and to future data, rapid field tests are recommended for a nationwide prevalence survey.
AB - Background: There is limited information regarding the prevalence of hepatitis B in Lao PDR, where the hepatitis disease burden is substantial. Thus, reliable seroprevalence data is needed for the disease, based on probability sampling. Methods: A stratified, multistage, cluster sampling survey of hepatitis B surface antigen (HBsAg) positivity among children aged 5-9 years and their mothers aged 15-45 years was conducted. Participants were selected randomly from the central region of Lao PDR via probability-proportional-to-size sampling. Blood samples were collected onto filter paper and subsequently analyzed using a chemiluminescent microparticle immunoassay. Results: A total of 911 mother-and-child pairs were collected; the seroprevalence of HBsAg was estimated to be 2.1% (95% confidence interval 0.8-3.4%) among children and 4.1% (95% confidence interval 2.6-5.5%) in their mothers after taking into account the sampling design and the weight of each sample. The children's HBsAg positivity was positively associated with maternal infection and being born in a non-health facility, while the maternal infection status was not associated with any background characteristic. Conclusions: Lao PDR has a relatively lower HBsAg prevalence in the general population compared to surrounding countries. To ensure comparability to other countries and to future data, rapid field tests are recommended for a nationwide prevalence survey.
KW - Chemiluminescent microparticle immunoassay
KW - Dried blood spots
KW - Hepatitis B
KW - Probability sampling
KW - Seroprevalence
KW - Southeast Asia
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U2 - 10.1016/j.ijid.2015.04.020
DO - 10.1016/j.ijid.2015.04.020
M3 - Article
C2 - 25957815
AN - SCOPUS:84930616786
SN - 1201-9712
VL - 36
SP - 21
EP - 26
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -
