Short-chain fatty acid signaling pathways in bovine mammary epithelial cells

Tomo Yonezawa, Satoshi Haga, Yosuke Kobayashi, Kazuo Katoh, Yoshiaki Obara

    Research output: Contribution to journalArticlepeer-review

    34 Citations (Scopus)


    GPR41 and 43 have recently been identified as G-protein-coupled cell-surface receptors for short-chain fatty acids (SCFAs). Bovine orthologs of GPR41 and 43 (bGPR41 and 43) mRNA were detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and various lactation stages of bovine mammary gland. Acetate and propionate caused an increase in intracellular Ca2+ concentrations in these cells that was blocked by the treatment with pertussis toxin (PTX). SCFAs significantly reduced forskolin-induced cAMP concentrations in these cells. The phosphorylation of mitogen-activated protein kinase (MAPK) p38 was selectively increased by SCFAs. The downstream substrate heat shock protein 27 (HSP27) was also phosphorylated by SCFAs at Ser-78 and -82, but not -15. These results suggest that bGPR41 mainly, but not bGPR43, mediate SCFA signaling in mammary epithelial cells and thereby plays some important role in mammary gland.

    Original languageEnglish
    Pages (from-to)30-36
    Number of pages7
    JournalRegulatory Peptides
    Issue number1-3
    Publication statusPublished - 2009 Feb 25


    • Mammary epithelial cell
    • Receptors for short-chain fatty acids
    • Short-chain fatty acids

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Endocrinology
    • Clinical Biochemistry
    • Cellular and Molecular Neuroscience


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