TY - JOUR
T1 - Simple and sensitive quantitation method for mevalonic acid in plasma using gas chromatography/mass spectrometry
AU - Ishihama, Yasushi
AU - Mano, Nariyasu
AU - Oda, Yoshiya
AU - Hiyoshi, Hironobu
AU - Ikuta, Hironori
AU - Yoshida, Yutaka
PY - 1994/5
Y1 - 1994/5
N2 - The combination of gas chromatography and mass spectrometry using a polar capillary column and isobutane chemical ionization made it possible to determine mevalonic acid (MVA) as the lactone at subnanogram levels using 1 mL of plasma. The pretreatment procedure consisted of only three steps, namely lactonization, washing with chloroform and liquid/liquid extraction. This simple, rapid and sensitive method, having good precision and accuracy, is useful for evaluating the change of plasma MVA, which is well correlated with whole‐body cholesterol biosynthesis.
AB - The combination of gas chromatography and mass spectrometry using a polar capillary column and isobutane chemical ionization made it possible to determine mevalonic acid (MVA) as the lactone at subnanogram levels using 1 mL of plasma. The pretreatment procedure consisted of only three steps, namely lactonization, washing with chloroform and liquid/liquid extraction. This simple, rapid and sensitive method, having good precision and accuracy, is useful for evaluating the change of plasma MVA, which is well correlated with whole‐body cholesterol biosynthesis.
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U2 - 10.1002/rcm.1290080507
DO - 10.1002/rcm.1290080507
M3 - Article
C2 - 8025334
AN - SCOPUS:0028437267
SN - 0951-4198
VL - 8
SP - 377
EP - 380
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 5
ER -