Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry

Takanori Hishinuma; Kaori Suzuki; Hiroaki Yamaguchi; Hatsushi Yamagishi; Tomoyuki Koike; Shuichi Ohara; Toru Shimosegawa; Nariyasu Mano; Junichi Goto

doi:10.1016/j.jchromb.2008.05.034

Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry

Takanori Hishinuma, Kaori Suzuki, Hiroaki Yamaguchi, Hatsushi Yamagishi, Tomoyuki Koike, Shuichi Ohara, Toru Shimosegawa, Nariyasu Mano, Junichi Goto

Tohoku University

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20 μL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1 mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25 ng/mL. A good linear response was observed for each analyte (from 0.25 ng to 2.5 μg/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies.

Original language	English
Pages (from-to)	38-45
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	870
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2008.05.034
Publication status	Published - 2008 Jul 1

Keywords

Determination
Human
Lansoprazole
LC/MS/MS
Rabeprazole
Serum

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10.1016/j.jchromb.2008.05.034

Cite this

Hishinuma, T., Suzuki, K., Yamaguchi, H., Yamagishi, H., Koike, T., Ohara, S., Shimosegawa, T., Mano, N., & Goto, J. (2008). Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 870(1), 38-45. https://doi.org/10.1016/j.jchromb.2008.05.034

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abstract = "A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20 μL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1 mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25 ng/mL. A good linear response was observed for each analyte (from 0.25 ng to 2.5 μg/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies.",

keywords = "Determination, Human, Lansoprazole, LC/MS/MS, Rabeprazole, Serum",

author = "Takanori Hishinuma and Kaori Suzuki and Hiroaki Yamaguchi and Hatsushi Yamagishi and Tomoyuki Koike and Shuichi Ohara and Toru Shimosegawa and Nariyasu Mano and Junichi Goto",

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doi = "10.1016/j.jchromb.2008.05.034",

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Hishinuma, T, Suzuki, K, Yamaguchi, H, Yamagishi, H, Koike, T, Ohara, S, Shimosegawa, T, Mano, N & Goto, J 2008, 'Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 870, no. 1, pp. 38-45. https://doi.org/10.1016/j.jchromb.2008.05.034

Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry. / Hishinuma, Takanori; Suzuki, Kaori; Yamaguchi, Hiroaki et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 870, No. 1, 01.07.2008, p. 38-45.

Research output: Contribution to journal › Article › peer-review

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T1 - Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry

AU - Hishinuma, Takanori

AU - Suzuki, Kaori

AU - Yamaguchi, Hiroaki

AU - Yamagishi, Hatsushi

AU - Koike, Tomoyuki

AU - Ohara, Shuichi

AU - Shimosegawa, Toru

AU - Mano, Nariyasu

AU - Goto, Junichi

PY - 2008/7/1

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N2 - A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20 μL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1 mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25 ng/mL. A good linear response was observed for each analyte (from 0.25 ng to 2.5 μg/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies.

AB - A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20 μL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1 mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25 ng/mL. A good linear response was observed for each analyte (from 0.25 ng to 2.5 μg/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies.

KW - Determination

KW - Human

KW - Lansoprazole

KW - LC/MS/MS

KW - Rabeprazole

KW - Serum

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Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry

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Keywords

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