Simulation analysis of positional relationship between embryoid bodies and sensors on an LSI-based amperometric device for electrochemical imaging of alkaline phosphatase activity

Yusuke Kanno; Kosuke Ino; Kumi Y. Inoue; Atsushi Suda; Ryota Kunikata; Masahki Matsudaira; Hitoshi Shiku; Tomokazu Matsue

doi:10.2116/analsci.31.715

Simulation analysis of positional relationship between embryoid bodies and sensors on an LSI-based amperometric device for electrochemical imaging of alkaline phosphatase activity

Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.

Original language	English
Pages (from-to)	715-719
Number of pages	5
Journal	Analytical Sciences
Volume	31
Issue number	7
DOIs	https://doi.org/10.2116/analsci.31.715
Publication status	Published - 2015

Keywords

Cell analysis
Electrochemical imaging
LSI-based amperometric chip device
Simulation analysis

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10.2116/analsci.31.715

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title = "Simulation analysis of positional relationship between embryoid bodies and sensors on an LSI-based amperometric device for electrochemical imaging of alkaline phosphatase activity",

abstract = "In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.",

keywords = "Cell analysis, Electrochemical imaging, LSI-based amperometric chip device, Simulation analysis",

author = "Yusuke Kanno and Kosuke Ino and Inoue, {Kumi Y.} and Atsushi Suda and Ryota Kunikata and Masahki Matsudaira and Hitoshi Shiku and Tomokazu Matsue",

note = "Publisher Copyright: {\textcopyright} 2015 The Japan Society for Analytical Chemistry.",

year = "2015",

doi = "10.2116/analsci.31.715",

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T1 - Simulation analysis of positional relationship between embryoid bodies and sensors on an LSI-based amperometric device for electrochemical imaging of alkaline phosphatase activity

AU - Kanno, Yusuke

AU - Ino, Kosuke

AU - Inoue, Kumi Y.

AU - Suda, Atsushi

AU - Kunikata, Ryota

AU - Matsudaira, Masahki

AU - Shiku, Hitoshi

AU - Matsue, Tomokazu

PY - 2015

Y1 - 2015

N2 - In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.

AB - In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.

KW - Cell analysis

KW - Electrochemical imaging

KW - LSI-based amperometric chip device

KW - Simulation analysis

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Simulation analysis of positional relationship between embryoid bodies and sensors on an LSI-based amperometric device for electrochemical imaging of alkaline phosphatase activity

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Keywords

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