Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry

Hiroki Tsukamoto; Takanori Hishinuma; Tsuyoshi Mikkaichi; Hironori Nakamura; Tohru Yamazaki; Yoshihisa Tomioka; Michinao Mizugaki

doi:10.1016/S1570-0232(02)00220-9

Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry

Hiroki Tsukamoto, Takanori Hishinuma, Tsuyoshi Mikkaichi, Hironori Nakamura, Tohru Yamazaki, Yoshihisa Tomioka, Michinao Mizugaki

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

We have developed a simultaneous quantification method for prostaglandin (PG) E₂, PGD₂, PGF_2α, 8-epi-PGF_2α, 6-keto-PGF_1α and thromboxane (TX) B₂. Using [3,3,4,4-²H₄]PGE₂, [3,3,4,4-²H₄]PGD₂, [3,3,4,4-²H₄]8-epi-PGF_2α, [3,3,4,4-²H₄]PGF_2α, [3,3,4,4-²H₄]6-keto-PGF_1α and [18,18,19,19-²H₄]TXB₂ as internal standards (I.S.), the eicosanoids and their I.S. were simultaneously extracted by solid-phase extraction from cell-cultured medium, derivatized to methyl ester/methoxim/tert.-butyldimethylsilyl ether derivatives and analyzed using gas chromatography-mass spectrometry in the selected ion monitoring mode. The accuracy for the added eicosanoids ranged from 92 to 113%, and coefficients of variation ranged from 0.1 to 12.2%. Increased eicosanoids in RAW264.7 and U937 cells stimulated by lipopolysaccharide were suppressed by NS-398 and indometacin. This simultaneous quantification method can be applied routinely for assaying eicosanoids in vitro.

Original language	English
Pages (from-to)	205-214
Number of pages	10
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	774
Issue number	2
DOIs	https://doi.org/10.1016/S1570-0232(02)00220-9
Publication status	Published - 2002 Jul 15

Keywords

Isoprostane
Prostaglandins
Thromboxane

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

Access to Document

10.1016/S1570-0232(02)00220-9

Cite this

Tsukamoto, H., Hishinuma, T., Mikkaichi, T., Nakamura, H., Yamazaki, T., Tomioka, Y., & Mizugaki, M. (2002). Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 774(2), 205-214. https://doi.org/10.1016/S1570-0232(02)00220-9

Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry. / Tsukamoto, Hiroki; Hishinuma, Takanori; Mikkaichi, Tsuyoshi et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 774, No. 2, 15.07.2002, p. 205-214.

Research output: Contribution to journal › Article › peer-review

Tsukamoto, H, Hishinuma, T, Mikkaichi, T, Nakamura, H, Yamazaki, T, Tomioka, Y & Mizugaki, M 2002, 'Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 774, no. 2, pp. 205-214. https://doi.org/10.1016/S1570-0232(02)00220-9

Tsukamoto H, Hishinuma T, Mikkaichi T, Nakamura H, Yamazaki T, Tomioka Y et al. Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2002 Jul 15;774(2):205-214. doi: 10.1016/S1570-0232(02)00220-9

@article{89b519553229450e9745959788f2aae0,

title = "Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry",

abstract = "We have developed a simultaneous quantification method for prostaglandin (PG) E2, PGD2, PGF2α, 8-epi-PGF2α, 6-keto-PGF1α and thromboxane (TX) B2. Using [3,3,4,4-2H4]PGE2, [3,3,4,4-2H4]PGD2, [3,3,4,4-2H4]8-epi-PGF2α, [3,3,4,4-2H4]PGF2α, [3,3,4,4-2H4]6-keto-PGF1α and [18,18,19,19-2H4]TXB2 as internal standards (I.S.), the eicosanoids and their I.S. were simultaneously extracted by solid-phase extraction from cell-cultured medium, derivatized to methyl ester/methoxim/tert.-butyldimethylsilyl ether derivatives and analyzed using gas chromatography-mass spectrometry in the selected ion monitoring mode. The accuracy for the added eicosanoids ranged from 92 to 113%, and coefficients of variation ranged from 0.1 to 12.2%. Increased eicosanoids in RAW264.7 and U937 cells stimulated by lipopolysaccharide were suppressed by NS-398 and indometacin. This simultaneous quantification method can be applied routinely for assaying eicosanoids in vitro.",

keywords = "Isoprostane, Prostaglandins, Thromboxane",

author = "Hiroki Tsukamoto and Takanori Hishinuma and Tsuyoshi Mikkaichi and Hironori Nakamura and Tohru Yamazaki and Yoshihisa Tomioka and Michinao Mizugaki",

year = "2002",

month = jul,

day = "15",

doi = "10.1016/S1570-0232(02)00220-9",

language = "English",

volume = "774",

pages = "205--214",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry

AU - Tsukamoto, Hiroki

AU - Hishinuma, Takanori

AU - Mikkaichi, Tsuyoshi

AU - Nakamura, Hironori

AU - Yamazaki, Tohru

AU - Tomioka, Yoshihisa

AU - Mizugaki, Michinao

PY - 2002/7/15

Y1 - 2002/7/15

N2 - We have developed a simultaneous quantification method for prostaglandin (PG) E2, PGD2, PGF2α, 8-epi-PGF2α, 6-keto-PGF1α and thromboxane (TX) B2. Using [3,3,4,4-2H4]PGE2, [3,3,4,4-2H4]PGD2, [3,3,4,4-2H4]8-epi-PGF2α, [3,3,4,4-2H4]PGF2α, [3,3,4,4-2H4]6-keto-PGF1α and [18,18,19,19-2H4]TXB2 as internal standards (I.S.), the eicosanoids and their I.S. were simultaneously extracted by solid-phase extraction from cell-cultured medium, derivatized to methyl ester/methoxim/tert.-butyldimethylsilyl ether derivatives and analyzed using gas chromatography-mass spectrometry in the selected ion monitoring mode. The accuracy for the added eicosanoids ranged from 92 to 113%, and coefficients of variation ranged from 0.1 to 12.2%. Increased eicosanoids in RAW264.7 and U937 cells stimulated by lipopolysaccharide were suppressed by NS-398 and indometacin. This simultaneous quantification method can be applied routinely for assaying eicosanoids in vitro.

AB - We have developed a simultaneous quantification method for prostaglandin (PG) E2, PGD2, PGF2α, 8-epi-PGF2α, 6-keto-PGF1α and thromboxane (TX) B2. Using [3,3,4,4-2H4]PGE2, [3,3,4,4-2H4]PGD2, [3,3,4,4-2H4]8-epi-PGF2α, [3,3,4,4-2H4]PGF2α, [3,3,4,4-2H4]6-keto-PGF1α and [18,18,19,19-2H4]TXB2 as internal standards (I.S.), the eicosanoids and their I.S. were simultaneously extracted by solid-phase extraction from cell-cultured medium, derivatized to methyl ester/methoxim/tert.-butyldimethylsilyl ether derivatives and analyzed using gas chromatography-mass spectrometry in the selected ion monitoring mode. The accuracy for the added eicosanoids ranged from 92 to 113%, and coefficients of variation ranged from 0.1 to 12.2%. Increased eicosanoids in RAW264.7 and U937 cells stimulated by lipopolysaccharide were suppressed by NS-398 and indometacin. This simultaneous quantification method can be applied routinely for assaying eicosanoids in vitro.

KW - Isoprostane

KW - Prostaglandins

KW - Thromboxane

UR - http://www.scopus.com/inward/record.url?scp=0037099576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037099576&partnerID=8YFLogxK

U2 - 10.1016/S1570-0232(02)00220-9

DO - 10.1016/S1570-0232(02)00220-9

M3 - Article

C2 - 12076690

AN - SCOPUS:0037099576

SN - 1570-0232

VL - 774

SP - 205

EP - 214

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Simultaneous quantification of prostaglandins, isoprostane and thromboxane in cell-cultured medium using gas chromatography-mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this