TY - JOUR
T1 - Simultaneous quantification of seven prostanoids using liquid chromatography/tandem mass spectrometry
T2 - The effects of arachidonic acid on prostanoid production in mouse bone marrow-derived mast cells
AU - Hishinuma, Takanori
AU - Suzuki, Kaori
AU - Saito, Masayoshi
AU - Yamaguchi, Hiroaki
AU - Suzuki, Naoto
AU - Tomioka, Yoshihisa
AU - Kaneko, Izumi
AU - Ono, Masao
AU - Goto, Junichi
N1 - Funding Information:
A part of this research was supported by Grant-in-Aid for Strategic Japanese-Swedish Cooperative Program on “Multidisciplinary Bio” from Japan Science and Technology Agency, Grant-in-Aid for Scientific Research(B) and Exploratory Research from Ministry of Education, Japan.
PY - 2007/6
Y1 - 2007/6
N2 - We have developed a method for the simultaneous estimation of the levels of the prostanoids 6-keto prostaglandin (PG) Flα, PGB2, PGD2, PGE2, PGF2α, PGJ2, and thromboxane (TX) B2 in blood- or serum-containing medium using liquid chromatography-tandem mass spectrometry. These prostanoids and their deuterium derivatives, which were used as internal standards, were subjected to solid-phase extraction using Empore C18 HD disk cartridges and analyzed in the selected reaction-monitoring mode. A linear response curve starting at 10 pg of prostanoid/tube was observed for each prostanoid. The accuracy of the method was demonstrated with samples containing known amounts of the prostanoids. Furthermore, we used this method to analyze the prostanoids produced in mouse bone marrow-derived mast cells stimulated with arachidonic acid, which resulted in the production of PGD2, PGE2, PGF2α, and TXB2. The results suggest that this simultaneous quantification method is useful for the analysis of the production of biomedically important prostanoids.
AB - We have developed a method for the simultaneous estimation of the levels of the prostanoids 6-keto prostaglandin (PG) Flα, PGB2, PGD2, PGE2, PGF2α, PGJ2, and thromboxane (TX) B2 in blood- or serum-containing medium using liquid chromatography-tandem mass spectrometry. These prostanoids and their deuterium derivatives, which were used as internal standards, were subjected to solid-phase extraction using Empore C18 HD disk cartridges and analyzed in the selected reaction-monitoring mode. A linear response curve starting at 10 pg of prostanoid/tube was observed for each prostanoid. The accuracy of the method was demonstrated with samples containing known amounts of the prostanoids. Furthermore, we used this method to analyze the prostanoids produced in mouse bone marrow-derived mast cells stimulated with arachidonic acid, which resulted in the production of PGD2, PGE2, PGF2α, and TXB2. The results suggest that this simultaneous quantification method is useful for the analysis of the production of biomedically important prostanoids.
UR - http://www.scopus.com/inward/record.url?scp=34447650584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34447650584&partnerID=8YFLogxK
U2 - 10.1016/j.plefa.2007.04.005
DO - 10.1016/j.plefa.2007.04.005
M3 - Article
C2 - 17590322
AN - SCOPUS:34447650584
SN - 0952-3278
VL - 76
SP - 321
EP - 329
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
IS - 6
ER -