@article{1d3850883b404d0c9f5c1818dbab51ce,
title = "Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans",
abstract = "To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving C. elegans by combining a microfluidic device and a tracking stage. Here we detail the protocol for the experiment, with an example of behavioral and neural responses of nematodes to salt concentration changes. For complete details on the use and execution of this protocol, please refer to Sato et al. (2021).",
keywords = "Behavior, Microscopy, Model Organisms, Neuroscience",
author = "Hirofumi Sato and Hirofumi Kunitomo and Xianfeng Fei and Koichi Hashimoto and Yuichi Iino",
note = "Funding Information: We thank the Caenorhabditis Genetics Center and the laboratory of Cornelia I. Bargmann for providing strains and the design of microfluidic devices. We also thank members of the Y.I. laboratory for helpful comments and advice with the experiments. This work was supported by the Core Research for Evolutional Science and Technology program (CREST) “Creation of Fundamental Technologies for Understanding and Control of Biosystem Dynamics,” Grant JPMJCR12W1, of the Japan Science and Technology Agency; Japan Society for the Promotion of Science KAKENHI Grant-in-Aid for Scientific Research (S) Grant JP17H06113; Grants-in-Aid for Innovative Area “Artificial Intelligence and Brain Science” (19H04980) and Japan Society for the Promotion of Science KAKENHI Grant-in-Aid for Early-Career Scientists Grant 19K16286, The salt Science Foundation No. 2043, and Center for Integrative Science of Human Behavior (CiSHuB). H.S. H.K. and Y.I. designed experiments; H.S. performed experiments; H.K. X.F. and K.H. contributed new reagents/analytic tools; H.S. analyzed data; and H.S. H.K. and Y.I. wrote the paper. The authors declare no competing interests. Funding Information: We thank the Caenorhabditis Genetics Center and the laboratory of Cornelia I. Bargmann for providing strains and the design of microfluidic devices. We also thank members of the Y.I. laboratory for helpful comments and advice with the experiments. This work was supported by the Core Research for Evolutional Science and Technology program (CREST) “Creation of Fundamental Technologies for Understanding and Control of Biosystem Dynamics,” Grant JPMJCR12W1 , of the Japan Science and Technology Agency ; Japan Society for the Promotion of Science KAKENHI Grant-in-Aid for Scientific Research (S) Grant JP17H06113 ; Grants-in-Aid for Innovative Area {"} Artificial Intelligence and Brain Science {"} ( 19H04980 ) and Japan Society for the Promotion of Science KAKENHI Grant-in-Aid for Early-Career Scientists Grant 19K16286 , The salt Science Foundation No. 2043 , and Center for Integrative Science of Human Behavior (CiSHuB). Publisher Copyright: {\textcopyright} 2021",
year = "2021",
month = dec,
day = "17",
doi = "10.1016/j.xpro.2021.101011",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}