Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision

Naoko Ogawa; Hiromasa Oku; Koichi Hashimoto; Masatoshi Ishikawa

Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision

Naoko Ogawa, Hiromasa Oku, Koichi Hashimoto, Masatoshi Ishikawa

INF - System Information Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

15 Citations (Scopus)

Abstract

A novel system for measurement of motile microorganism galvanotaxis using high-speed vision is presented. Our goal is to construct a smart microsystem composed of many controlled microorganisms called "Organized Bio-Modules" (OBM). The OEM system utilizes galvanotaxis (intrinsic reaction to electrical stimulus) of microorganisms for actuation. For evaluation of taxis, continuous observation of a moving single cell in a sufficiently large working area without fixation is needed. Using high-speed vision, we developed a system for continuous evaluation of galvanotaxis of freely swimming cells in a large area at the single-cell level. Experimental results demonstrate the continuous measurement of galvanotaxis of a Paramecium caudatum cell moving in a 3.5-mm-square area for 18 s with 1 μs precision.

Original language	English
Title of host publication	2004 2nd IEEE International Symposium on Biomedical Imaging
Subtitle of host publication	Macro to Nano
Pages	1331-1334
Number of pages	4
Publication status	Published - 2004
Event	2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano - Arlington, VA, United States Duration: 2004 Apr 15 → 2004 Apr 18

Publication series

Name	2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
Volume	2

Conference

Conference	2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
Country/Territory	United States
City	Arlington, VA
Period	04/4/15 → 04/4/18

Cite this

@inproceedings{cbbaa428cbca454b81495e5dd0eddaa0,

title = "Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision",

abstract = "A novel system for measurement of motile microorganism galvanotaxis using high-speed vision is presented. Our goal is to construct a smart microsystem composed of many controlled microorganisms called {"}Organized Bio-Modules{"} (OBM). The OEM system utilizes galvanotaxis (intrinsic reaction to electrical stimulus) of microorganisms for actuation. For evaluation of taxis, continuous observation of a moving single cell in a sufficiently large working area without fixation is needed. Using high-speed vision, we developed a system for continuous evaluation of galvanotaxis of freely swimming cells in a large area at the single-cell level. Experimental results demonstrate the continuous measurement of galvanotaxis of a Paramecium caudatum cell moving in a 3.5-mm-square area for 18 s with 1 μs precision.",

author = "Naoko Ogawa and Hiromasa Oku and Koichi Hashimoto and Masatoshi Ishikawa",

year = "2004",

language = "English",

isbn = "0780383885",

series = "2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano",

pages = "1331--1334",

booktitle = "2004 2nd IEEE International Symposium on Biomedical Imaging",

note = "2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano ; Conference date: 15-04-2004 Through 18-04-2004",

}

Ogawa, N, Oku, H, Hashimoto, K & Ishikawa, M 2004, Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision. in 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, vol. 2, pp. 1331-1334, 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, Arlington, VA, United States, 04/4/15.

Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision. / Ogawa, Naoko; Oku, Hiromasa; Hashimoto, Koichi et al.
2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. 2004. p. 1331-1334 (2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision

AU - Ogawa, Naoko

AU - Oku, Hiromasa

AU - Hashimoto, Koichi

AU - Ishikawa, Masatoshi

PY - 2004

Y1 - 2004

N2 - A novel system for measurement of motile microorganism galvanotaxis using high-speed vision is presented. Our goal is to construct a smart microsystem composed of many controlled microorganisms called "Organized Bio-Modules" (OBM). The OEM system utilizes galvanotaxis (intrinsic reaction to electrical stimulus) of microorganisms for actuation. For evaluation of taxis, continuous observation of a moving single cell in a sufficiently large working area without fixation is needed. Using high-speed vision, we developed a system for continuous evaluation of galvanotaxis of freely swimming cells in a large area at the single-cell level. Experimental results demonstrate the continuous measurement of galvanotaxis of a Paramecium caudatum cell moving in a 3.5-mm-square area for 18 s with 1 μs precision.

AB - A novel system for measurement of motile microorganism galvanotaxis using high-speed vision is presented. Our goal is to construct a smart microsystem composed of many controlled microorganisms called "Organized Bio-Modules" (OBM). The OEM system utilizes galvanotaxis (intrinsic reaction to electrical stimulus) of microorganisms for actuation. For evaluation of taxis, continuous observation of a moving single cell in a sufficiently large working area without fixation is needed. Using high-speed vision, we developed a system for continuous evaluation of galvanotaxis of freely swimming cells in a large area at the single-cell level. Experimental results demonstrate the continuous measurement of galvanotaxis of a Paramecium caudatum cell moving in a 3.5-mm-square area for 18 s with 1 μs precision.

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M3 - Conference contribution

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T3 - 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano

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BT - 2004 2nd IEEE International Symposium on Biomedical Imaging

T2 - 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano

Y2 - 15 April 2004 through 18 April 2004

ER -

Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision

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