Small-angle neutron scattering study on short-chain phosphatidylcholine micelle in the presence of sorbitol

H. Takahashi; M. Imai; Y. Matsushita; I. Hatta

doi:10.1007/BF01189525

Small-angle neutron scattering study on short-chain phosphatidylcholine micelle in the presence of sorbitol

H. Takahashi, M. Imai, Y. Matsushita, I. Hatta

SCI - Physics

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Sorbitol is a natural cryoprotectant for some organisms. In order to reveal the mechanism of a protection some organisms against low temperatures, we studied the interaction between sorbitol and phosphatidylcholine (PC) which is one of major component of cell membranes. Small-angle neutron-scattering contrast variation method was used to characterize dihexanoyl-PC (dC(6)PC) micelles in the presence of sorbitol (2 M). As a result, the aggregation number for the dC(6)PC micelle and the volume per a single molecule in the micelle were estimated to be ∼, 32 and ∼ 640 Å³, respectively. By comparing these data and those for the dC(6)PC micelle in pure water (Lin et al., J Am Chem Soc 108:3499-3507), it was concluded that sorbitol reduces the contact area between the lipid and the aqueous phase. On the basis of this conclusion, we discuss the role of sorbitol for the ability of some organisms to survive in low temperatures.

Original language	English
Pages (from-to)	223-227
Number of pages	5
Journal	Progress in Colloid and Polymer Science
Volume	106
DOIs	https://doi.org/10.1007/BF01189525
Publication status	Published - 1997

Keywords

Contrast-variation method
Cryoprotectant
Micelle
Neutron scattering
Phosphatidylcholine
Sorbitol

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10.1007/BF01189525

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title = "Small-angle neutron scattering study on short-chain phosphatidylcholine micelle in the presence of sorbitol",

abstract = "Sorbitol is a natural cryoprotectant for some organisms. In order to reveal the mechanism of a protection some organisms against low temperatures, we studied the interaction between sorbitol and phosphatidylcholine (PC) which is one of major component of cell membranes. Small-angle neutron-scattering contrast variation method was used to characterize dihexanoyl-PC (dC(6)PC) micelles in the presence of sorbitol (2 M). As a result, the aggregation number for the dC(6)PC micelle and the volume per a single molecule in the micelle were estimated to be ∼, 32 and ∼ 640 {\AA}3, respectively. By comparing these data and those for the dC(6)PC micelle in pure water (Lin et al., J Am Chem Soc 108:3499-3507), it was concluded that sorbitol reduces the contact area between the lipid and the aqueous phase. On the basis of this conclusion, we discuss the role of sorbitol for the ability of some organisms to survive in low temperatures.",

keywords = "Contrast-variation method, Cryoprotectant, Micelle, Neutron scattering, Phosphatidylcholine, Sorbitol",

author = "H. Takahashi and M. Imai and Y. Matsushita and I. Hatta",

year = "1997",

doi = "10.1007/BF01189525",

language = "English",

volume = "106",

pages = "223--227",

journal = "Progress in Colloid and Polymer Science",

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TY - JOUR

T1 - Small-angle neutron scattering study on short-chain phosphatidylcholine micelle in the presence of sorbitol

AU - Takahashi, H.

AU - Imai, M.

AU - Matsushita, Y.

AU - Hatta, I.

PY - 1997

Y1 - 1997

N2 - Sorbitol is a natural cryoprotectant for some organisms. In order to reveal the mechanism of a protection some organisms against low temperatures, we studied the interaction between sorbitol and phosphatidylcholine (PC) which is one of major component of cell membranes. Small-angle neutron-scattering contrast variation method was used to characterize dihexanoyl-PC (dC(6)PC) micelles in the presence of sorbitol (2 M). As a result, the aggregation number for the dC(6)PC micelle and the volume per a single molecule in the micelle were estimated to be ∼, 32 and ∼ 640 Å3, respectively. By comparing these data and those for the dC(6)PC micelle in pure water (Lin et al., J Am Chem Soc 108:3499-3507), it was concluded that sorbitol reduces the contact area between the lipid and the aqueous phase. On the basis of this conclusion, we discuss the role of sorbitol for the ability of some organisms to survive in low temperatures.

AB - Sorbitol is a natural cryoprotectant for some organisms. In order to reveal the mechanism of a protection some organisms against low temperatures, we studied the interaction between sorbitol and phosphatidylcholine (PC) which is one of major component of cell membranes. Small-angle neutron-scattering contrast variation method was used to characterize dihexanoyl-PC (dC(6)PC) micelles in the presence of sorbitol (2 M). As a result, the aggregation number for the dC(6)PC micelle and the volume per a single molecule in the micelle were estimated to be ∼, 32 and ∼ 640 Å3, respectively. By comparing these data and those for the dC(6)PC micelle in pure water (Lin et al., J Am Chem Soc 108:3499-3507), it was concluded that sorbitol reduces the contact area between the lipid and the aqueous phase. On the basis of this conclusion, we discuss the role of sorbitol for the ability of some organisms to survive in low temperatures.

KW - Contrast-variation method

KW - Cryoprotectant

KW - Micelle

KW - Neutron scattering

KW - Phosphatidylcholine

KW - Sorbitol

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SN - 0340-255X

VL - 106

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EP - 227

JO - Progress in Colloid and Polymer Science

JF - Progress in Colloid and Polymer Science

ER -

Small-angle neutron scattering study on short-chain phosphatidylcholine micelle in the presence of sorbitol

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Keywords

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