Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

Mizuki Hino; Kazuki Kurogi; Masa Aki Okubo; Maki Murata-Hori; Hiroshi Hosoya

doi:10.1006/bbrc.2000.2553

Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

Mizuki Hino, Kazuki Kurogi, Masa Aki Okubo, Maki Murata-Hori, Hiroshi Hosoya

IRIDeS - Disaster Medical Science Division

Hiroshima University

Research output: Contribution to journal › Article › peer-review

88 Citations (Scopus)

Abstract

One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both α- and β-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	164-169
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	271
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.2000.2553
Publication status	Published - 2000 Apr 29

Keywords

HSP27
Microtubules
Mitotic spindle
Monoclonal antibody
Small heat shock protein
Tubulin

Access to Document

10.1006/bbrc.2000.2553

Cite this

@article{1957fae5c2ff48088b09954ba3c1e788,

title = "Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells",

abstract = "One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both α- and β-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. (C) 2000 Academic Press.",

keywords = "HSP27, Microtubules, Mitotic spindle, Monoclonal antibody, Small heat shock protein, Tubulin",

author = "Mizuki Hino and Kazuki Kurogi and Okubo, {Masa Aki} and Maki Murata-Hori and Hiroshi Hosoya",

note = "Funding Information: This work was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (to H. H.).",

year = "2000",

month = apr,

day = "29",

doi = "10.1006/bbrc.2000.2553",

language = "English",

volume = "271",

pages = "164--169",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

AU - Hino, Mizuki

AU - Kurogi, Kazuki

AU - Okubo, Masa Aki

AU - Murata-Hori, Maki

AU - Hosoya, Hiroshi

N1 - Funding Information: This work was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (to H. H.).

PY - 2000/4/29

Y1 - 2000/4/29

N2 - One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both α- and β-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. (C) 2000 Academic Press.

AB - One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both α- and β-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. (C) 2000 Academic Press.

KW - HSP27

KW - Microtubules

KW - Mitotic spindle

KW - Monoclonal antibody

KW - Small heat shock protein

KW - Tubulin

UR - http://www.scopus.com/inward/record.url?scp=0034728761&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034728761&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2000.2553

DO - 10.1006/bbrc.2000.2553

M3 - Article

C2 - 10777697

AN - SCOPUS:0034728761

SN - 0006-291X

VL - 271

SP - 164

EP - 169

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this