TY - JOUR
T1 - SMC6 is required for MMS-induced interchromosomal and sister chromatid recombinations in Saccharomyces cerevisiae
AU - Onoda, Fumitoshi
AU - Takeda, Masahiro
AU - Seki, Masayuki
AU - Maeda, Daisuke
AU - Tajima, Jun Ichi
AU - Ui, Ayako
AU - Yagi, Hideki
AU - Enomoto, Takemi
N1 - Funding Information:
We thank Dr. L. Hartwell, Dr. B. Demple, Dr. A.R. Lehmann, Dr. Y. Kikuchi and Dr. H. Ikeda for strains and plasmids. This work was supported by Grants-in-Aid for Scientific Research and for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan, a Health Sciences Research Grant from Ministry of Health Labor and Welfare of Japan, and a grant from Takeda Science Foundation. M. Seki was supported in parts by The Mochida Memorial Foundation for Medical and Pharmaceutical Research.
PY - 2004/4/1
Y1 - 2004/4/1
N2 - SMC6 (RHC18) in Saccharomyces cerevisiae, which is a homologue of the Schizosaccharomyces pombe rad18+ gene and essential for cell viability, encodes a structural maintenance of chromosomes (SMC) family protein. In contrast to the rest of the SMC family of proteins, Smc1-Smc4, which are the components of cohesin or condensin, little is known about Smc6. In this study, we generated temperature sensitive (ts) smc6 mutants of budding yeast and characterized their properties. One ts-mutant, smc6-56, ceased growth soon after up-shift to a non-permissive temperature, arrested in the late S and G2/M phase, and gradually lost viability. smc6-56 cells at a permissive temperature showed a higher sensitivity than wild-type cells to various DNA damaging agents including methyl methanesulfonate (MMS). The rad52 smc6-56 double mutant showed a sensitivity to MMS similar to that of the rad52 single mutant, indicating that Smc6 is involved in a pathway that requires Rad52 to function. Moreover, no induction of interchromosomal recombination and sister chromatid recombination was observed in smc6-56 cells, which occurred in wild-type cells upon exposure to MMS.
AB - SMC6 (RHC18) in Saccharomyces cerevisiae, which is a homologue of the Schizosaccharomyces pombe rad18+ gene and essential for cell viability, encodes a structural maintenance of chromosomes (SMC) family protein. In contrast to the rest of the SMC family of proteins, Smc1-Smc4, which are the components of cohesin or condensin, little is known about Smc6. In this study, we generated temperature sensitive (ts) smc6 mutants of budding yeast and characterized their properties. One ts-mutant, smc6-56, ceased growth soon after up-shift to a non-permissive temperature, arrested in the late S and G2/M phase, and gradually lost viability. smc6-56 cells at a permissive temperature showed a higher sensitivity than wild-type cells to various DNA damaging agents including methyl methanesulfonate (MMS). The rad52 smc6-56 double mutant showed a sensitivity to MMS similar to that of the rad52 single mutant, indicating that Smc6 is involved in a pathway that requires Rad52 to function. Moreover, no induction of interchromosomal recombination and sister chromatid recombination was observed in smc6-56 cells, which occurred in wild-type cells upon exposure to MMS.
KW - rad18
KW - RAD52
KW - Recombination
KW - RHC18
KW - SMC6
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U2 - 10.1016/j.dnarep.2003.12.007
DO - 10.1016/j.dnarep.2003.12.007
M3 - Article
C2 - 15010319
AN - SCOPUS:1542269014
SN - 1568-7864
VL - 3
SP - 429
EP - 439
JO - DNA Repair
JF - DNA Repair
IS - 4
ER -