Solubilization and functional changes of biomolecules in ionic liquids

Masahiro Goto; Kojiro Shimojo; Kazunori Nakashima

Solubilization and functional changes of biomolecules in ionic liquids

Masahiro Goto, Kojiro Shimojo, Kazunori Nakashima

ENG - Chemical Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

This lecture reports on the protein transfer from an aqueous phase into ionic liquids (ILs) with dicyclohexano-18-crown-6 (DCH18C6), and the functional change of cytochrome c (Cyt-c) dissolved in ILs. Further, a protein is modified with comb-shaped poly (ethylene glycol), PM₁₃. PM₁₃ modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.

Original language	English
Pages	4176-4177
Number of pages	2
Publication status	Published - 2006
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 2006 Sept 20 → 2006 Sept 22

Other

Other	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	06/9/20 → 06/9/22

Keywords

Biotransformation /
Dissolution
Enzymatic reaction
Ionic liquids
Protein
Solubilization

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Solubilization and functional changes of biomolecules in ionic liquids",

abstract = "This lecture reports on the protein transfer from an aqueous phase into ionic liquids (ILs) with dicyclohexano-18-crown-6 (DCH18C6), and the functional change of cytochrome c (Cyt-c) dissolved in ILs. Further, a protein is modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.",

keywords = "Biotransformation /, Dissolution, Enzymatic reaction, Ionic liquids, Protein, Solubilization",

author = "Masahiro Goto and Kojiro Shimojo and Kazunori Nakashima",

year = "2006",

language = "English",

pages = "4176--4177",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

}

TY - CONF

T1 - Solubilization and functional changes of biomolecules in ionic liquids

AU - Goto, Masahiro

AU - Shimojo, Kojiro

AU - Nakashima, Kazunori

PY - 2006

Y1 - 2006

N2 - This lecture reports on the protein transfer from an aqueous phase into ionic liquids (ILs) with dicyclohexano-18-crown-6 (DCH18C6), and the functional change of cytochrome c (Cyt-c) dissolved in ILs. Further, a protein is modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.

AB - This lecture reports on the protein transfer from an aqueous phase into ionic liquids (ILs) with dicyclohexano-18-crown-6 (DCH18C6), and the functional change of cytochrome c (Cyt-c) dissolved in ILs. Further, a protein is modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.

KW - Biotransformation /

KW - Dissolution

KW - Enzymatic reaction

KW - Ionic liquids

KW - Protein

KW - Solubilization

UR - http://www.scopus.com/inward/record.url?scp=33846130053&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846130053&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33846130053

SP - 4176

EP - 4177

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Solubilization and functional changes of biomolecules in ionic liquids

Abstract

Other

Keywords

ASJC Scopus subject areas

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