Spatiotemporal sub-cellular biopatterning using an AFM-assisted electrochemical system

Soichiro Sekine, Hirokazu Kaji, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


The integration of electrochemical-based biolithography (ECBL) with an ordinary atomic force microscope (AFM) enables in situ lithography adjacent to a single, cultured cell, consequently allowing the morphological shape of the cell to be manipulated. The tip of a commercially available AFM cantilever was modified to serve as an electrode that could generate the oxidant HBrO for local, controlled etching of a cytophobic material (heparin or albumin) previously layered adjacent to a living cell. A NIH-3T3 fibroblast, initially confined to a patterned area, extended along a bioadhesive surface that had been newly exposed using the ECBL-AFM system.

Original languageEnglish
Pages (from-to)1781-1784
Number of pages4
JournalElectrochemistry Communications
Issue number9
Publication statusPublished - 2009 Sept


  • AFM
  • Lithography
  • Single cell


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