TY - JOUR
T1 - Species differences between chickens and rats in chemical properties of adipose tissue lipoprotein lipase
AU - Sato, Kan
AU - Akiba, Yukio
AU - Horiguchi, Masaaki
PY - 1997/11
Y1 - 1997/11
N2 - Chemical characterization of chicken and rat lipoprotein lipase (LPL) was carried out following purification of LPL. Molecular weight and isoelectric point of both purified enzymes were determined to be 60 KDa and pH 4, while optimum temperature and pH to yield the maximal activity were about 37°C and pH 8.5. Metallic ions, NaCl and protamine sulfate reduced, and heparin increased, both LPL activities. Michaelis constants for LPLs determined with triolein emulsion as the substrate were 0.98 and 1.57, and those of V(max) were 379.2 and 181.3, in chickens and rats, respectively. Triton WR-1339 caused mixed-type inhibition in rat, but inhibited chicken LPL noncompetitively. In LPLs of chickens and rats, values of K(i) were 66.7 and 36.4 with triolein emulsion as the substrate, and 832.4 and 66.0 with respective VLDL as the substrate. These results show species difference between chickens and rats in the affinity to lipoproteins of LPL and inhibition of LPL by Triton WR-1339.
AB - Chemical characterization of chicken and rat lipoprotein lipase (LPL) was carried out following purification of LPL. Molecular weight and isoelectric point of both purified enzymes were determined to be 60 KDa and pH 4, while optimum temperature and pH to yield the maximal activity were about 37°C and pH 8.5. Metallic ions, NaCl and protamine sulfate reduced, and heparin increased, both LPL activities. Michaelis constants for LPLs determined with triolein emulsion as the substrate were 0.98 and 1.57, and those of V(max) were 379.2 and 181.3, in chickens and rats, respectively. Triton WR-1339 caused mixed-type inhibition in rat, but inhibited chicken LPL noncompetitively. In LPLs of chickens and rats, values of K(i) were 66.7 and 36.4 with triolein emulsion as the substrate, and 832.4 and 66.0 with respective VLDL as the substrate. These results show species difference between chickens and rats in the affinity to lipoproteins of LPL and inhibition of LPL by Triton WR-1339.
KW - Chicken
KW - Lipoprotein lipase
KW - Rat
KW - Triton WR-1339
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UR - http://www.scopus.com/inward/citedby.url?scp=0031278830&partnerID=8YFLogxK
U2 - 10.1016/S0300-9629(97)00218-1
DO - 10.1016/S0300-9629(97)00218-1
M3 - Article
C2 - 9406449
AN - SCOPUS:0031278830
SN - 1095-6433
VL - 118
SP - 855
EP - 858
JO - Comparative Biochemistry and Physiology - A Physiology
JF - Comparative Biochemistry and Physiology - A Physiology
IS - 3
ER -