TY - JOUR
T1 - Specific Determination of Histamine in Fish by High-Performance Liquid Chromatography after Diazo Coupling
AU - Sato, Minoru
AU - Nakano, Toshiki
AU - Takeuchi, Masaaki
AU - Kumagai, Tsutomu
AU - Kanno, Nobuhiro
AU - Nagahisa, Eizou
AU - Sato, Yoshikazu
PY - 1995
Y1 - 1995
N2 - A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.
AB - A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.
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U2 - 10.1271/bbb.59.1208
DO - 10.1271/bbb.59.1208
M3 - Article
C2 - 7670179
AN - SCOPUS:0029340110
SN - 0916-8451
VL - 59
SP - 1208
EP - 1210
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -