Specific regions display altered grey matter volume in μ-opioid receptor knockout mice: MRI voxel-based morphometry

Kazumasu Sasaki, Akira Sumiyoshi, Hiroi Nonaka, Yoshiyuki Kasahara, Kazutaka Ikeda, F. Scott Hall, George R. Uhl, Masahiko Watanabe, Ryuta Kawashima, Ichiro Sora

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13 Citations (Scopus)


Background and Purpose μ Opioid receptor knockout (MOP-KO) mice display several behavioural differences from wild-type (WT) littermates including differential responses to nociceptive stimuli. Brain structural changes have been tied to behavioural alterations noted in transgenic mice with targeting of different genes. Hence, we assess the brain structure of MOP-KO mice.

Experimental Approach Magnetic resonance imaging (MRI) voxel-based morphometry (VBM) and histological methods were used to identify structural differences between extensively backcrossed MOP-KO mice and WT mice.

Key Results MOP-KO mice displayed robust increases in regional grey matter volume in olfactory bulb, several hypothalamic nuclei, periaqueductal grey (PAG) and several cerebellar areas, most confirmed by VBM analysis. The largest increases in grey matter volume were detected in the glomerular layer of the olfactory bulb, arcuate nucleus of hypothalamus, ventrolateral PAG (VLPAG) and cerebellar regions including paramedian and cerebellar lobules. Histological analyses confirm several of these results, with increased VLPAG cell numbers and increased thickness of the olfactory bulb granule cell layer and cerebellar molecular and granular cell layers.

Conclusions and Implications MOP deletion causes previously undescribed structural changes in specific brain regions, but not in all regions with high MOP receptor densities (e.g. thalamus, nucleus accumbens) or that exhibit adult neurogenesis (e.g. hippocampus). Volume differences in hypothalamus and PAG may reflect behavioural changes including hyperalgesia. Although the precise relationship between volume change and MOP receptor deletion was not determined from this study alone, these findings suggest that levels of MOP receptor expression may influence a broader range of neural structure and function in humans than previously supposed.

Original languageEnglish
Pages (from-to)654-667
Number of pages14
JournalBritish Journal of Pharmacology
Issue number2
Publication statusPublished - 2015 Jan


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