TY - JOUR
T1 - Specific ultraviolet-C irradiation energy for functionalization of titanium surface to increase osteoblastic cellular attachment
AU - Uchiyama, Hiroshi
AU - Yamada, Masahiro
AU - Ishizaki, Ken
AU - Sakurai, Kaoru
N1 - Funding Information:
This research was supported by Grant-in-Aid for Scientific Research (C) of Japan, 2012–2014 (Grant No. 24592932), Grant-in-Aid for Young Scientists (B) of Japan, 2012–2013 (24792114) and Oral Health Science Center Grant hrc8 from Tokyo Dental College, and by a Project for Private Universities: matching fund subsidy from MEXT of Japan, 2010–2012.
Funding Information:
The author thanks the research associate Eiko Watanabe of the Oral Health Science Center for her assistance with the evaluation of chemical composition by XPS. This research was supported by Grant-in-Aid for Scientific Research (C) of Japan, 2012-2014, Grant-in-Aid for Young Scientists (B) of Japan, 2012-2013 and Oral Health Science Center Grant hrc8 from Tokyo Dental College, and by a Project for Private Universities: matching fund subsidy from MEXT of Japan, 2010–2012.
PY - 2014/5
Y1 - 2014/5
N2 - Purpose: The objective of this in vitro study was to examine the influence of the total energy of ultraviolet-C preirradiation on the number and morphology of osteoblastic cells attached to turned or acid-etched titanium surfaces, and physicochemical properties of the surface. Materials and methods: Rat bone marrow-derived osteoblasts were incubated with turned or acid-etched titanium disks preirradiated with ultraviolet-C at 1 or 3 mW/cm2, resulting in total energies of 10, 100, 250, 400, 500, 600, 750, or 1000 J/cm2. Osteoblast attachment to the surface was evaluated using the WST-1 assay. Physicochemical changes of the titanium were evaluated by measuring water wettability and X-ray photoelectron spectroscopy analysis. Results: Number of attached cells was greater on turned or acid-etched surface preirradiated with 500 or 750 J/cm2 of 3 mW/cm2 ultraviolet-C than on the nonirradiated surface, respectively. However, the further irradiation energy did not increase the numbers on both types of the surfaces. These phenomena were also seen on the surfaces preirradiated at different ultraviolet-C intensities. Ultraviolet-C irradiation induced superhydrophilicity on both types of surface even with the less irradiation energy. The amount of carbon on ultraviolet-C preirradiated titanium surfaces decreased gradually with an increase in the total irradiation energy. Conclusion: Specific ultraviolet-C energy used to irradiate turned or acid-etched surfaces increased the number of osteoblastic cells attached to each of the surface. This was canceled by overirradiation, despite maintenance of both the acquired superhydrophilicity and the accompanying reduction in carbon on each surface.
AB - Purpose: The objective of this in vitro study was to examine the influence of the total energy of ultraviolet-C preirradiation on the number and morphology of osteoblastic cells attached to turned or acid-etched titanium surfaces, and physicochemical properties of the surface. Materials and methods: Rat bone marrow-derived osteoblasts were incubated with turned or acid-etched titanium disks preirradiated with ultraviolet-C at 1 or 3 mW/cm2, resulting in total energies of 10, 100, 250, 400, 500, 600, 750, or 1000 J/cm2. Osteoblast attachment to the surface was evaluated using the WST-1 assay. Physicochemical changes of the titanium were evaluated by measuring water wettability and X-ray photoelectron spectroscopy analysis. Results: Number of attached cells was greater on turned or acid-etched surface preirradiated with 500 or 750 J/cm2 of 3 mW/cm2 ultraviolet-C than on the nonirradiated surface, respectively. However, the further irradiation energy did not increase the numbers on both types of the surfaces. These phenomena were also seen on the surfaces preirradiated at different ultraviolet-C intensities. Ultraviolet-C irradiation induced superhydrophilicity on both types of surface even with the less irradiation energy. The amount of carbon on ultraviolet-C preirradiated titanium surfaces decreased gradually with an increase in the total irradiation energy. Conclusion: Specific ultraviolet-C energy used to irradiate turned or acid-etched surfaces increased the number of osteoblastic cells attached to each of the surface. This was canceled by overirradiation, despite maintenance of both the acquired superhydrophilicity and the accompanying reduction in carbon on each surface.
KW - Cell-material interaction
KW - hydrocarbon
KW - hydrophilicity
KW - osseointegration
KW - surface property
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U2 - 10.1177/0885328213511678
DO - 10.1177/0885328213511678
M3 - Article
C2 - 24287983
AN - SCOPUS:84899013728
SN - 0885-3282
VL - 28
SP - 1419
EP - 1429
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
IS - 9
ER -