Sprouting from on-chip vascular networks by a pressure-driven flow

Masamune Nakayama, Yuji Nashimoto, Itsuki Kunita, Akiko Nakamasu, Yu Suke Torisawa, Hirofumi Shintaku, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We quantitatively evaluated how vascular networks reconstructed in a microfluidic device were remodeled under a pressure-driven flow. We fabricated a PDMS device, and co-cultured human umbilical vein endothelial cells (HUVECs) and normal human lung fibroblasts (hLFs) to spontaneously reconstruct vascular networks. After a pressure-driven flow (0-120 μL/h) was applied to the vascular networks for 24 hours, we found the number of angiogenic sprouting increasing with the increase of flow rate and perfusion time.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages429-430
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Angiogenic sprouting
  • Microfluidic device
  • Pressure-driven flow
  • Vascular network

ASJC Scopus subject areas

  • Control and Systems Engineering

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