STED microscopy – super-resolution bio-imaging utilizing a stimulated emission depletion

Kohei Otomo, Terumasa Hibi, Yuichi Kozawa, Tomomi Nemoto

Research output: Contribution to journalReview articlepeer-review

20 Citations (Scopus)


One of the most popular super-resolution microscopies that breaks the diffraction barrier is stimulated emission depletion (STED) microscopy. As the optical set-up of STED microscopy is based on a laser scanning microscopy (LSM) system, it potentially has several merits of LSM like confocal or two-photon excitation LSM. In this article, we first describe the principles of STED microscopy and then describe the features of our newly developed two-photon excitation STED microscopy. On the basis of our recent results and those of other researchers, we conclude by discussing future research and new technologies in this field.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalMicroscopy (Oxford, England)
Issue number4
Publication statusPublished - 2015 Aug 1


  • Liquid crystal devices
  • Optical vortex
  • Two-photon microscopy


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