Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

Naoyuki Sarai; Wataru Kagawa; Takashi Kinebuchi; Ako Kagawa; Kozo Tanaka; Kiyoshi Miyagawa; Shukuko Ikawa; Takehiko Shibata; Hitoshi Kurumizaka; Shigeyuki Yokoyama

doi:10.1093/nar/gkl562

Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

Naoyuki Sarai, Wataru Kagawa, Takashi Kinebuchi, Ako Kagawa, Kozo Tanaka, Kiyoshi Miyagawa, Shukuko Ikawa, Takehiko Shibata, Hitoshi Kurumizaka, Shigeyuki Yokoyama

IDAC - Division of Cancer Science

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.

Original language	English
Pages (from-to)	4429-4437
Number of pages	9
Journal	Nucleic Acids Research
Volume	34
Issue number	16
DOIs	https://doi.org/10.1093/nar/gkl562
Publication status	Published - 2006 Sept

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abstract = "The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.",

author = "Naoyuki Sarai and Wataru Kagawa and Takashi Kinebuchi and Ako Kagawa and Kozo Tanaka and Kiyoshi Miyagawa and Shukuko Ikawa and Takehiko Shibata and Hitoshi Kurumizaka and Shigeyuki Yokoyama",

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AU - Sarai, Naoyuki

AU - Kagawa, Wataru

AU - Kinebuchi, Takashi

AU - Kagawa, Ako

AU - Tanaka, Kozo

AU - Miyagawa, Kiyoshi

AU - Ikawa, Shukuko

AU - Shibata, Takehiko

AU - Kurumizaka, Hitoshi

AU - Yokoyama, Shigeyuki

PY - 2006/9

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N2 - The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.

AB - The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.

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Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

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