Stimulation of hydrogenase synthesis in free-living Bradyrhizobium japonicum by addition of nitrate and ammonium

Katsuhiko Fukai; Kiwamu Minamisawa; Teruo Asami

doi:10.1080/00380768.1990.10415001

Stimulation of hydrogenase synthesis in free-living Bradyrhizobium japonicum by addition of nitrate and ammonium

Katsuhiko Fukai, Kiwamu Minamisawa, Teruo Asami

LIF - Ecological Developmental Adaptability Life Sciences

Ibaraki University

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Free-living cells as well as bacteroids of Bradyrhizobium japonicum are able to oxidize H₂evolved from nitrogenase reaction. Low partial pressure of O₂in the presence of H₂and low concentration of carbon substrates were considered to be required for the expression of the uptake hydrogenase (Hup) activity in free-living B. japonicum (Maier et al. 1978). However, the Hup activity reported by Maier et al. (1979) was lower than that of bacteroids (McCrae et al. 1978). When we examined the conditions of derepression for higher Hup activity in free-living B. japonicum cells, we observed that the addition of NH₄C1 enhanced the Hup activity. Therefore, we decided to investigate the effects of nitrogen sources on the derepression of the Hup activity in free-living B. japonicum.

Original language	English
Pages (from-to)	345-347
Number of pages	3
Journal	Soil Science and Plant Nutrition
Volume	36
Issue number	2
DOIs	https://doi.org/10.1080/00380768.1990.10415001
Publication status	Published - 1990 Jun

Keywords

Bradyrhizobium japonicum
Derepression
Hydrogenase
Nitrate

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10.1080/00380768.1990.10415001

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title = "Stimulation of hydrogenase synthesis in free-living Bradyrhizobium japonicum by addition of nitrate and ammonium",

abstract = "Free-living cells as well as bacteroids of Bradyrhizobium japonicum are able to oxidize H2evolved from nitrogenase reaction. Low partial pressure of O2in the presence of H2and low concentration of carbon substrates were considered to be required for the expression of the uptake hydrogenase (Hup) activity in free-living B. japonicum (Maier et al. 1978). However, the Hup activity reported by Maier et al. (1979) was lower than that of bacteroids (McCrae et al. 1978). When we examined the conditions of derepression for higher Hup activity in free-living B. japonicum cells, we observed that the addition of NH4C1 enhanced the Hup activity. Therefore, we decided to investigate the effects of nitrogen sources on the derepression of the Hup activity in free-living B. japonicum.",

keywords = "Bradyrhizobium japonicum, Derepression, Hydrogenase, Nitrate",

author = "Katsuhiko Fukai and Kiwamu Minamisawa and Teruo Asami",

year = "1990",

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doi = "10.1080/00380768.1990.10415001",

language = "English",

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T1 - Stimulation of hydrogenase synthesis in free-living Bradyrhizobium japonicum by addition of nitrate and ammonium

AU - Fukai, Katsuhiko

AU - Minamisawa, Kiwamu

AU - Asami, Teruo

PY - 1990/6

Y1 - 1990/6

N2 - Free-living cells as well as bacteroids of Bradyrhizobium japonicum are able to oxidize H2evolved from nitrogenase reaction. Low partial pressure of O2in the presence of H2and low concentration of carbon substrates were considered to be required for the expression of the uptake hydrogenase (Hup) activity in free-living B. japonicum (Maier et al. 1978). However, the Hup activity reported by Maier et al. (1979) was lower than that of bacteroids (McCrae et al. 1978). When we examined the conditions of derepression for higher Hup activity in free-living B. japonicum cells, we observed that the addition of NH4C1 enhanced the Hup activity. Therefore, we decided to investigate the effects of nitrogen sources on the derepression of the Hup activity in free-living B. japonicum.

AB - Free-living cells as well as bacteroids of Bradyrhizobium japonicum are able to oxidize H2evolved from nitrogenase reaction. Low partial pressure of O2in the presence of H2and low concentration of carbon substrates were considered to be required for the expression of the uptake hydrogenase (Hup) activity in free-living B. japonicum (Maier et al. 1978). However, the Hup activity reported by Maier et al. (1979) was lower than that of bacteroids (McCrae et al. 1978). When we examined the conditions of derepression for higher Hup activity in free-living B. japonicum cells, we observed that the addition of NH4C1 enhanced the Hup activity. Therefore, we decided to investigate the effects of nitrogen sources on the derepression of the Hup activity in free-living B. japonicum.

KW - Bradyrhizobium japonicum

KW - Derepression

KW - Hydrogenase

KW - Nitrate

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Stimulation of hydrogenase synthesis in free-living Bradyrhizobium japonicum by addition of nitrate and ammonium

Abstract

Keywords

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