TY - JOUR
T1 - Strigolactone perception and deactivation by a hydrolase receptor DWARF14
AU - Seto, Yoshiya
AU - Yasui, Rei
AU - Kameoka, Hiromu
AU - Tamiru, Muluneh
AU - Cao, Mengmeng
AU - Terauchi, Ryohei
AU - Sakurada, Akane
AU - Hirano, Rena
AU - Kisugi, Takaya
AU - Hanada, Atsushi
AU - Umehara, Mikihisa
AU - Seo, Eunjoo
AU - Akiyama, Kohki
AU - Burke, Jason
AU - Takeda-Kamiya, Noriko
AU - Li, Weiqiang
AU - Hirano, Yoshinori
AU - Hakoshima, Toshio
AU - Mashiguchi, Kiyoshi
AU - Noel, Joseph P.
AU - Kyozuka, Junko
AU - Yamaguchi, Shinjiro
N1 - Funding Information:
This work was supported by JSPS/MEXT KAKENHI (Grant Nos, 24780117, 24114010, and 17H06474), the Program for Promotion of Basic and Applied Research for Innovations in Bio-Oriented Industry, and the Core Research for Evolutional Science and Technology (CREST) by JST (Grant No, JPMJCR13B1). Y.S. was a JSPS Postdoctoral Fellow for Research Abroad. We thank Dr. Mitsunori Seo for providing the yeast expression vector, pYES ADHpro.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an α/β-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14 D218A catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.
AB - The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an α/β-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14 D218A catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.
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U2 - 10.1038/s41467-018-08124-7
DO - 10.1038/s41467-018-08124-7
M3 - Article
C2 - 30643123
AN - SCOPUS:85060044052
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 191
ER -