Strong inhibitory effects of curcumin and its demethoxy analog on Escherichia coli ATP synthase F1 sector

Mizuki Sekiya, Eiko Chiba, Momoe Satoh, Hiroyuki Yamakoshi, Yoshiharu Iwabuchi, Masamitsu Futai, Mayumi Nakanishi-Matsui

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Curcumin, a dietary phytopolyphenol isolated from a perennial herb (Curcuma longa), is a well-known compound effective for bacterial infections and tumors, and also as an antioxidant. In this study, we report the inhibitory effects of curcumin and its analogs on the Escherichia coli ATP synthase F1 sector. A structure-activity relationship study indicated the importance of 4'-hydroxy groups and a β-diketone moiety for the inhibition. The 3'-demethoxy analog (DMC) inhibited F1 more strongly than curcumin did. Furthermore, these compounds inhibited E. coli growth through oxidative phosphorylation, consistent with their effects on ATPase activity. These results suggest that the two compounds affected bacterial growth through inhibition of ATP synthase. Derivatives including bis(arylmethylidene)acetones (C5 curcuminoids) exhibited only weak activity toward ATPase and bacterial growth.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume70
DOIs
Publication statusPublished - 2014 Sept

Keywords

  • ATP synthase
  • Curcumin
  • F-ATPase

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