@article{ddfa339ec6ed44dfa1ab801a6ddd01f0,
title = "Structural Basis of Karrikin and Non-natural Strigolactone Perception in Physcomitrella patens",
abstract = "In plants, strigolactones are perceived by the dual receptor-hydrolase DWARF14 (D14). D14 belongs to the superfamily of α/β hydrolases and is structurally similar to the karrikin receptor KARRIKIN INSENSITIVE 2 (KAI2). The moss Physcomitrella patens is an ideal model system for studying this receptor family, because it includes 11 highly related family members with unknown ligand specificity. We present the crystal structures of three Physcomitrella D14/KAI2-like proteins and describe a loop-based mechanism that leads to a permanent widening of the hydrophobic substrate gorge. We have identified protein clades that specifically perceive the karrikin KAR1 and the non-natural strigolactone isomer (−)-5-deoxystrigol in a highly stereoselective manner.",
keywords = "Physcomitrella patens, karrikin, strigolactone, α/β hydrolases",
author = "Marco B{\"u}rger and Kiyoshi Mashiguchi and Lee, {Hyun Jee} and Misaki Nakano and Kodai Takemoto and Yoshiya Seto and Shinjiro Yamaguchi and Joanne Chory",
note = "Funding Information: We thank the staff at Advanced Light Source at the Berkeley Center for Structural Biology for assistance with X-ray data collection. The Berkeley Center for Structural Biology is supported in part by the NIH, National Institute of General Medical Sciences, and the Howard Hughes Medical Institute. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences of the U.S. Department of Energy (contract DE-AC02-05CH11231 ). This work was further supported by the Mass Spectrometry Core of the Salk Institute with funding from NIH-NCI CCSG ( P30 014195 ) and the Helmsley Center for Genomic Medicine . Financial support for this work came from the Howard Hughes Medical Institute (to J.C.). We thank James Moresco, Jolene Diedrich, and Antonio Michel Pinto for technical support. We thank the laboratory of Prof. Kohki Akiyama (Graduate School of Life and Environmental Sciences, Osaka Prefecture University) for providing rac -carlactone. J.C. is an investigator of the Howard Hughes Medical Institute. Funding Information: We thank the staff at Advanced Light Source at the Berkeley Center for Structural Biology for assistance with X-ray data collection. The Berkeley Center for Structural Biology is supported in part by the NIH, National Institute of General Medical Sciences, and the Howard Hughes Medical Institute. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences of the U.S. Department of Energy (contract DE-AC02-05CH11231). This work was further supported by the Mass Spectrometry Core of the Salk Institute with funding from NIH-NCI CCSG (P30 014195) and the Helmsley Center for Genomic Medicine. Financial support for this work came from the Howard Hughes Medical Institute (to J.C.). We thank James Moresco, Jolene Diedrich, and Antonio Michel Pinto for technical support. We thank the laboratory of Prof. Kohki Akiyama (Graduate School of Life and Environmental Sciences, Osaka Prefecture University) for providing rac-carlactone. J.C. is an investigator of the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2019 The Authors",
year = "2019",
month = jan,
day = "22",
doi = "10.1016/j.celrep.2019.01.003",
language = "English",
volume = "26",
pages = "855--865.e5",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "4",
}