Structural studies of enzyme-based microfluidic biofuel cells

Makoto Togo, Akimasa Takamura, Tatsuya Asai, Hirokazu Kaji, Matsuhiko Nishizawa

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88 Citations (Scopus)


An enzyme-based glucose/O2 biofuel cell was constructed within a microfluidic channel to study the influence of electrode configuration and fluidic channel height on cell performance. The cell was composed of a bilirubin oxidase (BOD)-adsorbed O2 cathode and a glucose anode prepared by co-immobilization of glucose dehydrogenase (GDH), diaphorase (Dp) and VK3-pendant poly-l-lysine. The consumption of O2 at the upstream cathode protected the downstream anode from interfering O2 molecules, and consequently improved the cell performance (maximum cell current) ca. 10% for the present cell. The cell performance was also affected by the channel height. The output current and power of a 0.1 mm-height cell was significantly less than those of a 1 mm-height cell because of the depletion of O2, as determined by the shape of the E-I curve at the cathode. On the other hand, the volume density of current and power was several times higher for the narrower cell.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalJournal of Power Sources
Issue number1
Publication statusPublished - 2008 Mar 15


  • Biofuel cell
  • Diaphorase
  • Glucose dehydrogenase
  • Microfluidics
  • Vitamin K


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