Structure determination and evolution of the chicken cDNA and gene encoding prepropancreatic polypeptide

Koji Nata, Takako Sugimoto, Kazuhiro Kohri, Hiroshi Hidaka, Eiji Hattori, Hiroshi Yamamoto, Hideto Yonekura, Hiroshi Okamoto

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13 Citations (Scopus)


We have previously demonstrated that the C-terminal regions of the rat and human pancreatic polypeptide (PPP) precursors exhibit a high degree of divergence, whereas the N-terminal regions are highly conserved. This blend of structural conservation and divergence in the precursors appears to be caused by splice junction sliding and translational frameshift in the 3'-region of the PPP gene [Yonekura et al., J. Biol. Chem. 263 (1988) 2990-2997]. In the present study, we determined the nucleotide (nt) sequences of the chicken PPP (cPPP) cDNA and gene, and compared them with those of the mammals. In cPPP, the C-terminal region of the precursor is quite heterologous with respect to the rat (rPPP) and human (hPPP) precursors, and this heterogeneity is accentuated by the large deletion in exon 3 of cPPP. Furthermore, mutational accumulation during evolution caused the structural organization of the 3'-region of cPPP to change; cPPP is terminated in exon 3, whereas rPPP and hPPP are terminated in exon 4. Thus, our previous observation regarding the possibility of 'mosaic evolution' [Yamamoto et al., J. Biol. Chem. 261 (1986) 6156-6159] of PPP has been extended and confirmed by this study. Available evidence suggests that 'mosaic evolution' is a phenomenon unique to PPP, and not to the genes encoding the other members of the PPP family, neuropeptide-Y and peptide-YY.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
Issue number2
Publication statusPublished - 1993 Aug 25


  • cloning
  • exon
  • intron
  • Mosaic evolution
  • neuropeptide-Y
  • nucleotide sequence
  • peptide-YY
  • recombinant DNA


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