Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes

Koji Yonekura; Chikashi Toyoshima

doi:10.1016/S0304-3991(00)00009-7

Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes

Koji Yonekura, Chikashi Toyoshima

IMRAM - Division of Organic- and Biomaterials Research

The University of Tokyo

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A set of programs has been developed for averaging the data from tubular crystals belonging to different helical classes. This was done either by (i) cutting out molecules constituting a unit cell from density maps, and aligning and averaging them in real space; (ii) transforming the densities in a unit cell to layer-line data according to a (possibly artificial) helical symmetry, aligning and averaging them in reciprocal space. These methods were applied to tubular crystals of Ca²⁺-ATPase. Either method worked well and substantially improved the data quality. Transforming the reconstructed images to the layer-line data has many advantages and is essential for fully exploiting the power of averaging. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	15-28
Number of pages	14
Journal	Ultramicroscopy
Volume	84
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3991(00)00009-7
Publication status	Published - 2000 Jul

Keywords

Ca-ATPase
Cryo-electron microscopy
Real-space averaging
Tubular crystal

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10.1016/S0304-3991(00)00009-7

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title = "Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes",

abstract = "A set of programs has been developed for averaging the data from tubular crystals belonging to different helical classes. This was done either by (i) cutting out molecules constituting a unit cell from density maps, and aligning and averaging them in real space; (ii) transforming the densities in a unit cell to layer-line data according to a (possibly artificial) helical symmetry, aligning and averaging them in reciprocal space. These methods were applied to tubular crystals of Ca2+-ATPase. Either method worked well and substantially improved the data quality. Transforming the reconstructed images to the layer-line data has many advantages and is essential for fully exploiting the power of averaging. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Ca-ATPase, Cryo-electron microscopy, Real-space averaging, Tubular crystal",

author = "Koji Yonekura and Chikashi Toyoshima",

note = "Funding Information: We thank Kazutoshi Tani for some of the programs used in this study and Dr Keiichi Namba for his program on the conversion of density map to [24] . This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (to C.T.).",

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T1 - Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes

AU - Yonekura, Koji

AU - Toyoshima, Chikashi

N1 - Funding Information: We thank Kazutoshi Tani for some of the programs used in this study and Dr Keiichi Namba for his program on the conversion of density map to [24] . This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (to C.T.).

PY - 2000/7

Y1 - 2000/7

N2 - A set of programs has been developed for averaging the data from tubular crystals belonging to different helical classes. This was done either by (i) cutting out molecules constituting a unit cell from density maps, and aligning and averaging them in real space; (ii) transforming the densities in a unit cell to layer-line data according to a (possibly artificial) helical symmetry, aligning and averaging them in reciprocal space. These methods were applied to tubular crystals of Ca2+-ATPase. Either method worked well and substantially improved the data quality. Transforming the reconstructed images to the layer-line data has many advantages and is essential for fully exploiting the power of averaging. Copyright (C) 2000 Elsevier Science B.V.

AB - A set of programs has been developed for averaging the data from tubular crystals belonging to different helical classes. This was done either by (i) cutting out molecules constituting a unit cell from density maps, and aligning and averaging them in real space; (ii) transforming the densities in a unit cell to layer-line data according to a (possibly artificial) helical symmetry, aligning and averaging them in reciprocal space. These methods were applied to tubular crystals of Ca2+-ATPase. Either method worked well and substantially improved the data quality. Transforming the reconstructed images to the layer-line data has many advantages and is essential for fully exploiting the power of averaging. Copyright (C) 2000 Elsevier Science B.V.

KW - Ca-ATPase

KW - Cryo-electron microscopy

KW - Real-space averaging

KW - Tubular crystal

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JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 1-2

ER -

Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes

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Keywords

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