TY - JOUR
T1 - Structure of the complete elongation complex of RNA polymerase II with basal factors
AU - Ehara, Haruhiko
AU - Yokoyama, Takeshi
AU - Shigematsu, Hideki
AU - Yokoyama, Shigeyuki
AU - Shirouzu, Mikako
AU - Sekine, Shun Ichi
N1 - Funding Information:
We thank Y. Tomabechi, T. Uchikubo-Kamo, and T. Osanai for technical assistance. The synchrotron radiation experiments were performed at BL41XU of SPring-8 with the approval of the Japan Synchrotron Radiation Research Institute (proposal nos. 2014B1265, 2015B2040, 2016A2526, and 2016B2526) and at NE3A of the Photon Factory with the approval of the Photon Factory Program Advisory Committee (proposal no. 2015G520). We thank H. Okumura and K. Hasegawa for assistance with data collection at SPring-8. This work was supported in part by the Japan Society for the Promotion of Science KAKENHI grant numbers JP15H04344 (to S.S.) and JP15H01656 (to H.S.), the Platform Project for Supporting Drug Discovery and Life Science Research funded by the Japan Agency for Medical Research and Development, and the RIKEN Pioneering Project, Dynamic Structural Biology. The atomic coordinates and structure factors for the EC bound with Elf1 and Spt5 KOW5 have been deposited in the Protein Data Bank (PDB ID 5XOG). The cryo-EM density map and the atomic coordinates for the EC bound with Spt4/5 and TFIIS were deposited in the Electron Microscopy (EM) Data Bank (accession code EMD-6747) and the Protein Data Bank (PDB ID 5XON), respectively.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - In the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex for processive transcription. Here we report the structure of the Pol II elongation complex bound with the basal elongation factors Spt4/5, Elf1, and TFIIS. Spt4/5 (the Spt4/Spt5 complex) and Elf1 modify a wide area of the Pol II surface. Elf1 bridges the Pol II central cleft, completing a "DNA entry tunnel" for downstream DNA. Spt4 and the Spt5 NGN and KOW1 domains encircle the upstream DNA, constituting a "DNA exit tunnel." The Spt5 KOW4 and KOW5 domains augment the "RNA exit tunnel," directing the exiting nascent RNA. Thus, the elongation complex establishes a completely different transcription and regulation platform from that of the initiation complexes.
AB - In the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex for processive transcription. Here we report the structure of the Pol II elongation complex bound with the basal elongation factors Spt4/5, Elf1, and TFIIS. Spt4/5 (the Spt4/Spt5 complex) and Elf1 modify a wide area of the Pol II surface. Elf1 bridges the Pol II central cleft, completing a "DNA entry tunnel" for downstream DNA. Spt4 and the Spt5 NGN and KOW1 domains encircle the upstream DNA, constituting a "DNA exit tunnel." The Spt5 KOW4 and KOW5 domains augment the "RNA exit tunnel," directing the exiting nascent RNA. Thus, the elongation complex establishes a completely different transcription and regulation platform from that of the initiation complexes.
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U2 - 10.1126/science.aan8552
DO - 10.1126/science.aan8552
M3 - Article
C2 - 28775211
AN - SCOPUS:85027019690
SN - 0036-8075
VL - 357
SP - 921
EP - 924
JO - Science
JF - Science
IS - 6354
ER -