A cloned rat gro gene encoding the neutrophil chemo-attractant Gro was isolated from a λCharon4A rat genomic library, and the nucleotide (nt) sequence of a 2500-bp fragment encompassing the coding region and 3'- and 5'-flanking regions was determined. The gene consisted of four exons separated by three introns. The transcription start point was determined by primer-extension analysis and found to be a G located 72 nt upstream from the start codon. The 5'- flanking region of the gene contained a 'TATA'-like structure and an NF-κB-binding sequence.
|Number of pages||2|
|Publication status||Published - 1993 Apr 30|
- interleukin-8 family
- transcription start point
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