Structures of genes encoding phospholipase A2 inhibitors from the serum of Trimeresurus flavoviridis snake

Ikuo Nobuhisa, Masanobu Deshimaru, Takahito Chijiwa, Kin Ichi Nakashima, Tomohisa Ogawa, Yasuyuki Shimohigashi, Yasuyuki Fukumaki, Shosaku Hattori, Hiroshi Kihara, Motonori Ohno

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Inhibitors (PLIs) against snake venom gland phospholipases A2 (PLA2s) have been found in their sera. A cDNA encoding a PLI from Trimeresurus flavoviridis (Tf, habu snake, Crotalinae) serum, cPLI-A, was isolated from the Tf liver cDNA library and sequenced. Northern blot analysis with cPLI-A showed that PLIs are expressed only in liver. Genes for PLIs, gPLI-A and gPLI-B, were isolated from the Tf genomic DNA library and their nucleotide (nt) sequences were determined. The genes consisted of four exons and three introns, and exon 4 encoded the carbohydrate recognition domain (CRD)-like motif. Comparison of the nt sequences between gPLI-A and gPLI-B showed that these genes are highly homologous, including introns, except that exon 3 is rich in nonsynonymous nt substitutions which are almost four times as frequent as synonymous nt substitutions. This evolutionary feature of PLI genes is different from that of venom gland PLA2 isozyme genes in which nonsynonymous nt substitutions are spread over the entire mature protein-coding region.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
Issue number1
Publication statusPublished - 1997 May 20
Externally publishedYes


  • Carbohydrate recognition domain
  • Crotalinae snake
  • Gene structure
  • Molecular evolution

ASJC Scopus subject areas

  • Genetics


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