TY - JOUR
T1 - Structures of genes encoding phospholipase A2 inhibitors from the serum of Trimeresurus flavoviridis snake
AU - Nobuhisa, Ikuo
AU - Deshimaru, Masanobu
AU - Chijiwa, Takahito
AU - Nakashima, Kin Ichi
AU - Ogawa, Tomohisa
AU - Shimohigashi, Yasuyuki
AU - Fukumaki, Yasuyuki
AU - Hattori, Shosaku
AU - Kihara, Hiroshi
AU - Ohno, Motonori
N1 - Funding Information:
This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.
PY - 1997/5/20
Y1 - 1997/5/20
N2 - Inhibitors (PLIs) against snake venom gland phospholipases A2 (PLA2s) have been found in their sera. A cDNA encoding a PLI from Trimeresurus flavoviridis (Tf, habu snake, Crotalinae) serum, cPLI-A, was isolated from the Tf liver cDNA library and sequenced. Northern blot analysis with cPLI-A showed that PLIs are expressed only in liver. Genes for PLIs, gPLI-A and gPLI-B, were isolated from the Tf genomic DNA library and their nucleotide (nt) sequences were determined. The genes consisted of four exons and three introns, and exon 4 encoded the carbohydrate recognition domain (CRD)-like motif. Comparison of the nt sequences between gPLI-A and gPLI-B showed that these genes are highly homologous, including introns, except that exon 3 is rich in nonsynonymous nt substitutions which are almost four times as frequent as synonymous nt substitutions. This evolutionary feature of PLI genes is different from that of venom gland PLA2 isozyme genes in which nonsynonymous nt substitutions are spread over the entire mature protein-coding region.
AB - Inhibitors (PLIs) against snake venom gland phospholipases A2 (PLA2s) have been found in their sera. A cDNA encoding a PLI from Trimeresurus flavoviridis (Tf, habu snake, Crotalinae) serum, cPLI-A, was isolated from the Tf liver cDNA library and sequenced. Northern blot analysis with cPLI-A showed that PLIs are expressed only in liver. Genes for PLIs, gPLI-A and gPLI-B, were isolated from the Tf genomic DNA library and their nucleotide (nt) sequences were determined. The genes consisted of four exons and three introns, and exon 4 encoded the carbohydrate recognition domain (CRD)-like motif. Comparison of the nt sequences between gPLI-A and gPLI-B showed that these genes are highly homologous, including introns, except that exon 3 is rich in nonsynonymous nt substitutions which are almost four times as frequent as synonymous nt substitutions. This evolutionary feature of PLI genes is different from that of venom gland PLA2 isozyme genes in which nonsynonymous nt substitutions are spread over the entire mature protein-coding region.
KW - Carbohydrate recognition domain
KW - Crotalinae snake
KW - Gene structure
KW - Molecular evolution
UR - http://www.scopus.com/inward/record.url?scp=0031007837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031007837&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(97)00024-3
DO - 10.1016/S0378-1119(97)00024-3
M3 - Article
C2 - 9210585
AN - SCOPUS:0031007837
SN - 0378-1119
VL - 191
SP - 31
EP - 37
JO - Gene
JF - Gene
IS - 1
ER -