TY - JOUR
T1 - Studies on substrate specificity at PR/p3 cleavage site of HTLV-1 protease
AU - Bang, Jeong Kyu
AU - Teruya, Kenta
AU - Aimoto, Saburo
AU - Konno, Hiroyuki
AU - Nosaka, Kazuto
AU - Tatsumi, Tadashi
AU - Akaji, Kenichi
PY - 2007/6
Y1 - 2007/6
N2 - Substrate specificities for recognition at the PR/p3 site of HTLV-1 protease were clarified using small libraries of substrate peptides. Specificities at P1 and P1′ positions were examined by parallel synthesis/digestion of synthetic peptides covering the PR/p3 site (KGPPVILPIQA). Specificities at P2 to P4 positions were examined by split and mix syntheses of olefin-peptide libraries containing the substrate sequence (PPVILPIQ). The solid-phase Horner-Emmons reaction was successfully applied to syntheses of multi-component substrates for library preparation. From the digestion of substrate peptides by a chemically synthesized mutant of HTLV-1 protease (C2A HTLV-1 PR), it was found for the first time that the preference for Pro at the P1′ position and for Ile at the P2 position is unique for this enzyme.
AB - Substrate specificities for recognition at the PR/p3 site of HTLV-1 protease were clarified using small libraries of substrate peptides. Specificities at P1 and P1′ positions were examined by parallel synthesis/digestion of synthetic peptides covering the PR/p3 site (KGPPVILPIQA). Specificities at P2 to P4 positions were examined by split and mix syntheses of olefin-peptide libraries containing the substrate sequence (PPVILPIQ). The solid-phase Horner-Emmons reaction was successfully applied to syntheses of multi-component substrates for library preparation. From the digestion of substrate peptides by a chemically synthesized mutant of HTLV-1 protease (C2A HTLV-1 PR), it was found for the first time that the preference for Pro at the P1′ position and for Ile at the P2 position is unique for this enzyme.
KW - Horner-Emmons reaction
KW - HTLV-1
KW - Solid-phase synthesis
KW - Substrate specificity
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U2 - 10.1007/s10989-006-9062-z
DO - 10.1007/s10989-006-9062-z
M3 - Article
AN - SCOPUS:34250014195
SN - 1573-3149
VL - 13
SP - 173
EP - 179
JO - International Journal of Peptide Research and Therapeutics
JF - International Journal of Peptide Research and Therapeutics
IS - 1-2
ER -