Study of specific interaction between transcription factor protein and DNA using colloid probe atomic force microscope

Yoshiki Nakata; Yuichiro Shimizu; Takehiro Suzuki; Masaya Fujita; Kazue Kurihara

Study of specific interaction between transcription factor protein and DNA using colloid probe atomic force microscope

Yoshiki Nakata, Yuichiro Shimizu, Takehiro Suzuki, Masaya Fujita, Kazue Kurihara

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to conference › Paper › peer-review

Abstract

Transcription factor protein, Spo0A-DB, of Bacillus Subtilis recognize base sequence TGTCGAA called 0Abox. We measured interactions between Spo0A-DB and DNA at various Spo0A-DB densities using colloid probe atomic force microscope. For this interaction measurement, we found the optimum density of Spo0A-DB which corresponded to the closest packing. At excess adsorbed conditions, Spo0A-DB isn't in an appropriate orientation or the DNA binding site of SpoOA-DB is blocked by others, and at smaller densities, influences of the bottom chelate layer is increased.

Original language	English
Pages	1874
Number of pages	1
Publication status	Published - 2006
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 2006 May 24 → 2006 May 26

Conference

Conference	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	06/5/24 → 06/5/26

Keywords

Bacillus Subtilis
Colloid probe atomic force microscope
Interaction between protein and DNA
Spo0A-DB

Cite this

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title = "Study of specific interaction between transcription factor protein and DNA using colloid probe atomic force microscope",

abstract = "Transcription factor protein, Spo0A-DB, of Bacillus Subtilis recognize base sequence TGTCGAA called 0Abox. We measured interactions between Spo0A-DB and DNA at various Spo0A-DB densities using colloid probe atomic force microscope. For this interaction measurement, we found the optimum density of Spo0A-DB which corresponded to the closest packing. At excess adsorbed conditions, Spo0A-DB isn't in an appropriate orientation or the DNA binding site of SpoOA-DB is blocked by others, and at smaller densities, influences of the bottom chelate layer is increased.",

keywords = "Bacillus Subtilis, Colloid probe atomic force microscope, Interaction between protein and DNA, Spo0A-DB",

author = "Yoshiki Nakata and Yuichiro Shimizu and Takehiro Suzuki and Masaya Fujita and Kazue Kurihara",

year = "2006",

language = "English",

pages = "1874",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

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T1 - Study of specific interaction between transcription factor protein and DNA using colloid probe atomic force microscope

AU - Nakata, Yoshiki

AU - Shimizu, Yuichiro

AU - Suzuki, Takehiro

AU - Fujita, Masaya

AU - Kurihara, Kazue

PY - 2006

Y1 - 2006

N2 - Transcription factor protein, Spo0A-DB, of Bacillus Subtilis recognize base sequence TGTCGAA called 0Abox. We measured interactions between Spo0A-DB and DNA at various Spo0A-DB densities using colloid probe atomic force microscope. For this interaction measurement, we found the optimum density of Spo0A-DB which corresponded to the closest packing. At excess adsorbed conditions, Spo0A-DB isn't in an appropriate orientation or the DNA binding site of SpoOA-DB is blocked by others, and at smaller densities, influences of the bottom chelate layer is increased.

AB - Transcription factor protein, Spo0A-DB, of Bacillus Subtilis recognize base sequence TGTCGAA called 0Abox. We measured interactions between Spo0A-DB and DNA at various Spo0A-DB densities using colloid probe atomic force microscope. For this interaction measurement, we found the optimum density of Spo0A-DB which corresponded to the closest packing. At excess adsorbed conditions, Spo0A-DB isn't in an appropriate orientation or the DNA binding site of SpoOA-DB is blocked by others, and at smaller densities, influences of the bottom chelate layer is increased.

KW - Bacillus Subtilis

KW - Colloid probe atomic force microscope

KW - Interaction between protein and DNA

KW - Spo0A-DB

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M3 - Paper

AN - SCOPUS:33749847492

SP - 1874

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Study of specific interaction between transcription factor protein and DNA using colloid probe atomic force microscope

Abstract

Conference

Keywords

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